BMRB: 6360

IDENTIFICATION OF MINIMAL PEPTIDE SEQUENCE IN THE AMIDATED FRAGMENT 33-61 OF BOVINE a-HEMOGLBIN

Authors

Machado, A., Silva, F.D., Miranda, A., Daffre, S., Sforca, M.L., Spisni, A., Pertinhez, T.A., Miranda, M.M.

Assembly

40-61 bovine alpha-hemoglobin fragment

Entity

1. 40-61 bovine alpha-hemoglobin fragment (polymer, Thiol state: not present), 23 monomers, 2410.665 Da Detail

KTYFPHFDLS HGSAQVKGHG AKX

Formula weight

2410.665 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 75.8 %, Completeness (bb): 91.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	75.8 % (100 of 132)	75.8 % (100 of 132)
Backbone	91.3 % (42 of 46)	91.3 % (42 of 46)
Sidechain	67.4 % (58 of 86)	67.4 % (58 of 86)
Aromatic	50.0 % (10 of 20)	50.0 % (10 of 20)
Methyl	85.7 % (6 of 7)	85.7 % (6 of 7)

1. amidated fragment (40-61) of bovine alpha-hemoglobin

KTYFPHFDLS HGSAQVKGHG AKX

Show sample condition

Sample

Temperature 310 (±1) K, pH 4.0 (±0.1), Details SDS micelles

#	Name	Isotope labeling	Type	Concentration
1	amidated fragment (40-61) of bovine alpha-hemoglobin			1.0 mM

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Release date

2008-07-15

Citation

The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.TERESA., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 , Abstract

Related entities 1. 40-61 bovine alpha-hemoglobin fragment, : 1 : 175 : 44 entities Detail

Interaction partners 1. 40-61 bovine alpha-hemoglobin fragment, : 32 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail