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IDENTIFICATION OF MINIMAL PEPTIDE SEQUENCE IN THE AMIDATED FRAGMENT 33-61 OF BOVINE a-HEMOGLBIN
Authors
Machado, A., Silva, F.D., Miranda, A., Daffre, S., Sforca, M.L., Spisni, A., Pertinhez, T.A., Miranda, M.M.
Assembly
40-61 bovine alpha-hemoglobin fragment
Entity
1. 40-61 bovine alpha-hemoglobin fragment (polymer, Thiol state: not present), 23 monomers, 2410.665 Da Detail

KTYFPHFDLS HGSAQVKGHG AKX


Formula weight
2410.665 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.7 %, Completeness: 81.1 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

Total1H
All81.1 % (107 of 132)81.1 % (107 of 132)
Backbone95.3 % (41 of 43)95.3 % (41 of 43)
Sidechain74.2 % (66 of 89)74.2 % (66 of 89)
Aromatic50.0 % (10 of 20)50.0 % (10 of 20)
Methyl85.7 % (6 of 7)85.7 % (6 of 7)

1. amidated fragment (40-61) of bovine alpha-hemoglobin

KTYFPHFDLS HGSAQVKGHG AKX

Sample

Temperature 310 (±1) K, pH 4.0 (±0.1), Details SDS micelles


#NameIsotope labelingTypeConcentration
1amidated fragment (40-61) of bovine alpha-hemoglobin1.0 mM

Release date
2008-07-15
Citation
The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.TERESA., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 ,
Related entities 1. 40-61 bovine alpha-hemoglobin fragment, : 1 : 174 : 25 entities Detail
Interaction partners 1. 40-61 bovine alpha-hemoglobin fragment, : 30 interactors Detail
Experiments performed 4 experiments Detail
Keywords antimicrobial peptide, MINIMAL PEPTIDE SEQUENCE, STRUCTURAL REQUIREMENTS