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BMRB: 6391

2K2G

1H, 13C, and 15N chemical shift assignments for the catalytic domain of MMP-12

Authors

Markus, M.A., Dwyer, B., Wolfrom, S., Li, J., Li, W., Malakian, K., Wilhelm, J., Tsao, D.H.H.

Assembly

mmp12

Entity

1. mmp12 (polymer, Thiol state: not present), 165 monomers, 18342.38 Da Detail

MFREMPGGPV WRKHYITYRI NNYTPDMNRE DVDYAIRKAF QVWSNVTPLK FSKINTGMAD ILVVFARGAH GDFHAFDGKG GILAHAFGPG SGIGGDAHFD EDEFWTTHSG GTNLFLTAVH EIGHSLGLGH SSDPKAVMFP TYKYVDINTF RLSADDIRGI QSLYG

2. DSV (non-polymer), 363.451 Da

Total weight

18705.832 Da

Max. entity weight

18342.38 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 86.3 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.3 % (1633 of 1893)	83.9 % (815 of 971)	87.6 % (659 of 752)	93.5 % (159 of 170)
Backbone	95.1 % (928 of 976)	94.2 % (323 of 343)	96.4 % (458 of 475)	93.0 % (147 of 158)
Sidechain	79.7 % (846 of 1062)	78.3 % (492 of 628)	81.0 % (342 of 422)	100.0 % (12 of 12)
Aromatic	46.4 % (115 of 248)	46.8 % (58 of 124)	44.6 % (54 of 121)	100.0 % (3 of 3)
Methyl	100.0 % (160 of 160)	100.0 % (80 of 80)	100.0 % (80 of 80)

1. mmp12

MFREMPGGPV WRKHYITYRI NNYTPDMNRE DVDYAIRKAF QVWSNVTPLK FSKINTGMAD ILVVFARGAH GDFHAFDGKG GILAHAFGPG SGIGGDAHFD EDEFWTTHSG GTNLFLTAVH EIGHSLGLGH SSDPKAVMFP TYKYVDINTF RLSADDIRGI QSLYG

Show sample condition

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	nitrogen	external	indirect	0.101329

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	nitrogen	external	indirect	0.101329

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	nitrogen	external	indirect	0.101329

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	nitrogen	external	indirect	0.101329

Referencing offset: 0.12 ppm, Outliers: 3 Detail

Release date

2005-04-07

Citation

1H, 13C, and 15N assignments of MMP-12, a key protease implicated in lung tissue remodeling
Markus, M.A., Dwyer, B., Wolfrom, S., Li, J., Li, W., Malakian, K., Wilhelm, J., Tsao, D.H.H.
J. Biomol. NMR (2005), 31, 260-260, PubMed 15803400 , DOI 10.1007/s10858-005-0181-1 , Abstract

Related entities 1. mmp12, : 1 : 82 : 196 entities Detail

Interaction partners 1. mmp12, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 HNCO

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

2 HNCA

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

3 HNCACB

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

4 CBCA(CO)NH

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

5 HN(CA)CO

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

6 C(CO)NH

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

7 H(CCO)NH

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

8 HBHA(CO)NH

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

9 HCCH TOCSY

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

10 HACAHB

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

11 HNHB

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

12 1H13C HSQC

Instrument

Model Bruker DRX (600 MHz) Details Triple resonance, single-axis gradient, cryoprobe for most experiments.

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
1	mmp12	[U-15N]		0.2 mM
2	inhibitor			0.2 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C 15N labelled MMP-12 (with bound inhibitor).

#	Name	Isotope labeling	Type	Concentration
3	mmp12	[U-13C; U-15N]		0.7 mM
4	inhibitor			0.7 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 13C, 15N labelled MMP-12 (with bound inhibitor) in 2H2O.

#	Name	Isotope labeling	Concentration
5	mmp12	[U-13C; U-15N]	0.4 mM
6	inhibitor		0.4 mM
7	D2O		100 %

Sample #4

Pressure 1 (±0.1) atm, Temperature 298 (±1.0) K, pH 6.0 (±0.2), Details 10% 13C labelled MMP-12 with bound inhibitor.

#	Name	Isotope labeling	Type	Concentration
8	mmp12	[U-10% 13C]		0.55 mM
9	inhibitor			0.55 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6391_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6391_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	1812		100.0 (chain: 1, length: 165)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 165, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 663
  - ¹H chemical shifts: 980
  - ¹⁵N chemical shifts: 169
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords chemical shift assignments, macrophage elastase, matrix metalloprotease

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	752	663	88.2
¹H chemical shifts	971	816	84.0
¹⁵N chemical shifts	178	169	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	330	321	97.3
¹H chemical shifts	343	328	95.6
¹⁵N chemical shifts	158	150	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	422	342	81.0
¹H chemical shifts	628	488	77.7
¹⁵N chemical shifts	20	19	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	121	54	44.6
¹H chemical shifts	124	58	46.8
¹⁵N chemical shifts	3	3	100.0

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Found 1 Document (0.831 seconds)

BMRB: 6391

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. mmp12, : 1 : 82 : 196 entities Detail

Interaction partners 1. mmp12, : 1 interactors Detail

Experiments performed 12 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

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