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BMRB: 6399

1HH8

1H, 13C and 15N resonance assignments for the 24 kDa TPR domain in p67phox

Authors

Yoshida, S., Ogura, K., Yokochi, M., Yuzawa, S., Horiuchi, M., Morioka, H., Sumimoto, H., Inagaki, F.

Assembly

TPR domain in p67phox

Entity

1. TPR domain in p67phox (polymer, Thiol state: all free), 208 monomers, 23858.60 Da Detail

GAMGSMSLVE AISLWNEGVL AADKKDWKGA LDAFSAVQDP HSRICFNIGC MYTILKNMTE AEKAFTRSIN RDKHLAVAYF QRGMLYYQTE KYDLAIKDLK EALIQLRGNQ LIDYKILGLQ FKLFACEVLY NIAFMYAKKE EWKKAEEQLA LATSMKSEPR HSKIDKAMEC VWKQKLYEPV VIPVGKLFRP NERQVAQLAK KDYLGKAT

Formula weight

23858.6 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 52.2 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.2 % (1325 of 2539)	30.8 % (409 of 1328)	70.8 % (699 of 987)	96.9 % (217 of 224)
Backbone	83.2 % (1030 of 1238)	54.4 % (229 of 421)	98.0 % (602 of 614)	98.0 % (199 of 203)
Sidechain	32.8 % (492 of 1499)	19.8 % (180 of 907)	51.5 % (294 of 571)	85.7 % (18 of 21)
Aromatic	8.2 % (18 of 220)	8.2 % (9 of 110)	4.7 % (5 of 106)	100.0 % (4 of 4)
Methyl	78.6 % (184 of 234)	70.9 % (83 of 117)	86.3 % (101 of 117)

1. TPR

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.49 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.49 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.19 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1HH8, Strand ID: A Detail

Release date

2005-07-25

Citation

1H, 13C and 15N resonance assignments of the backbone and methyl groups of the 24 kDa tetratricopeptide repeat domain in p67(phox)
Yoshida, S., Ogura, K., Yokochi, M., Yuzawa, S., Horiuchi, M., Morioka, H., Sumimoto, H., Inagaki, F.
J. Biomol. NMR (2005), 32, 176-176, PubMed 16034671 , DOI 10.1007/s10858-005-5276-1 , Abstract

Related entities 1. TPR domain in p67phox, : 1 : 1 : 3 : 17 entities Detail

Interaction partners 1. TPR domain in p67phox, : 16 interactors Detail

More details for 16 interactors identified by 8 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

2 2D 1H-13C CT-HSQC

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

3 HNCO

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

4 HN(CA)CO

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

5 HNCA

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

6 HN(CO)CA

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

7 HN(CA)CB

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

8 HN(COCA)CB

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

9 C(CO)NH

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

10 H(CCO)NH

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

11 H(C)CH-TOCSY

Instrument

#1 Model Varian Unity-Plus or AS (600 MHz)

#2 Model Varian AS (500 MHz) Details equiupped with cold probe

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
1	TPR	[U-80%(fractional) 2H; U-98% 13C; U-98% 15N]	0.8 mM
2	Na-Pi		50 mM
3	NaCl		150 mM
4	DTT		1 mM
5	MgCl2		10 mM
6	EDTA		0.5 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.2), Details Val, Leu and Ile(d1) methyl 1H-, uniform 2H/13C/15N-labeled sample.

#	Name	Isotope labeling	Concentration
9	TPR	[NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N]	0.7 mM
10	Na-Pi		50 mM
11	NaCl		150 mM
12	DTT		1 mM
13	MgCl2		10 mM
14	EDTA		0.5 mM
15	H2O		90 %
16	D2O		10 %

null

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Found 1 Document (1.899 seconds)

BMRB: 6399

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. TPR domain in p67phox, : 1 : 1 : 3 : 17 entities Detail

Interaction partners 1. TPR domain in p67phox, : 16 interactors Detail

Experiments performed 11 experiments Detail

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