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Backbone and side chain chemical shift assignments of a TRAV14-3 mouse T cell receptor domain
Authors
Hu, J.
Assembly
TCR Va2.6Ja38
Entity
1. TCR Va2.6Ja38 (polymer, Thiol state: all disulfide bound), 114 monomers, 12689.08 Da Detail

MQQVRQSPQS LTVWEGETAI LNCSYENSAF DYFPWYQQFP GEGPALLISI LSVSNKKEDG RFTIFFNKRE KKLSLHIADS QPGDSATYFC AASASFGDNS KLIWGLGTSL VVNP


Formula weight
12689.08 Da
Entity Connection
disulfide 1 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing0:CYS23:SG1:CYS90:SG

Source organism
Mus musculus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 93.4 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All93.4 % (1252 of 1341)93.5 % (648 of 693)92.9 % (484 of 521)94.5 % (120 of 127)
Backbone98.0 % (651 of 664)98.2 % (218 of 222)98.5 % (329 of 334)96.3 % (104 of 108)
Sidechain90.3 % (707 of 783)91.3 % (430 of 471)89.1 % (261 of 293)84.2 % (16 of 19)
Aromatic60.5 % (92 of 152)60.5 % (46 of 76)58.9 % (43 of 73)100.0 % (3 of 3)
Methyl100.0 % (110 of 110)100.0 % (55 of 55)100.0 % (55 of 55)

1. TCR Va2.6 domain

MQQVRQSPQS LTVWEGETAI LNCSYENSAF DYFPWYQQFP GEGPALLISI LSVSNKKEDG RFTIFFNKRE KKLSLHIADS QPGDSATYFC AASASFGDNS KLIWGLGTSL VVNP

Sample #1

Temperature 300 (±1) K, pH 7.0 (±0.2)


#NameIsotope labelingTypeConcentration
1TCR Va2.6 domain[U-95% 15N]0.94 mM
Sample #2

Temperature 300 (±1) K, pH 7.0 (±0.2)


#NameIsotope labelingTypeConcentration
2TCR Va2.6 domain[U-95% 13C; U-95% 15N]0.91 mM

LACS Plot; CA
Referencing offset: 0.02 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.02 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.76 ppm, Outliers: 1 Detail
Release date
2005-01-13
Citation
Letter to the Editor: Backbone and side chain resonance assignments of a TRAV14-3 mouse T cell receptor domain
Hu, J., Plaksin, D., Margulies, D.H.
J. Biomol. NMR (2005), 31, 271-272, PubMed , DOI:
Related entities 1. TCR Va2.6Ja38, : 1 : 2 : 247 entities Detail
Experiments performed 21 experiments Detail
Keywords NMR assignments, TRAV14-3*02, TRAJ38*01 domain, T cell receptor