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FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY
Authors
Machado, A., Sforca, M.L., Miranda, A., Daffre, S., Spisni, A., Pertinhez, T.A., Miranda, M.M.
Assembly
amidated fragment 33-52 of bovine alpha-hemoglobin
Entity
1. amidated fragment 33-52 of bovine alpha-hemoglobin (polymer, Thiol state: not present), 21 monomers, 2327.570 Da Detail

FLSFPTTKTY FPHFDLSHGS X


Formula weight
2327.57 Da
Source organism
Bos taurus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.2 %, Completeness: 78.4 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

Total1H
All78.4 % (98 of 125)78.4 % (98 of 125)
Backbone94.7 % (36 of 38)94.7 % (36 of 38)
Sidechain71.3 % (62 of 87)71.3 % (62 of 87)
Aromatic42.9 % (12 of 28)42.9 % (12 of 28)
Methyl100.0 % (7 of 7)100.0 % (7 of 7)

1. amidated fragment 33-52

FLSFPTTKTY FPHFDLSHGS X

Sample

Temperature 310 (±1) K, pH 4.0 (±0.2)


#NameIsotope labelingTypeConcentration
1amidated fragment 33-521.0 mM
2sodium dodecyl sulfate (SDS)200 mM

Release date
2008-07-16
Citation 1
The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.TERESA., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 ,
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 ,
Related entities 1. amidated fragment 33-52 of bovine alpha-hemoglobin, : 1 : 185 : 14 entities Detail
Interaction partners 1. amidated fragment 33-52 of bovine alpha-hemoglobin, : 30 interactors Detail
Experiments performed 4 experiments Detail
Keywords antimicrobial peptide, MINIMAL PEPTIDE SEQUENCE, STRUCTURAL REQUIREMENTS