BMRB: 6414

FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY

Authors

Machado, A., Sforca, M.L., Miranda, A., Daffre, S., Spisni, A., Pertinhez, T.A., Miranda, M.M.

Assembly

amidated fragment 33-52 of bovine alpha-hemoglobin

Entity

1. amidated fragment 33-52 of bovine alpha-hemoglobin (polymer, Thiol state: not present), 21 monomers, 2327.570 Da Detail

FLSFPTTKTY FPHFDLSHGS X

Formula weight

2327.57 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 77.6 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	77.6 % (97 of 125)	77.6 % (97 of 125)
Backbone	94.9 % (37 of 39)	94.9 % (37 of 39)
Sidechain	69.8 % (60 of 86)	69.8 % (60 of 86)
Aromatic	42.9 % (12 of 28)	42.9 % (12 of 28)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. amidated fragment 33-52

FLSFPTTKTY FPHFDLSHGS X

Show sample condition

Sample

Temperature 310 (±1) K, pH 4.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	amidated fragment 33-52			1.0 mM
2	sodium dodecyl sulfate (SDS)			200 mM

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Release date

2008-07-16

Citation 1

The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.TERESA., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. amidated fragment 33-52 of bovine alpha-hemoglobin, : 1 : 186 : 31 entities Detail

Interaction partners 1. amidated fragment 33-52 of bovine alpha-hemoglobin, : 32 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail