Search

Three-disulfide variant of hen lysozyme: C30A/C115A
Authors
Yokota, A., Hirai, K., Miyauchi, H., Iimura, S., Noda, Y., Inoue, K., Akasaka, K., Tachibana, H., Segawa, S.
Assembly
hen egg white lysozyme
Entity
1. hen egg white lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14380.07 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV AAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRAKGTD VQAWIRGCRL


Formula weight
14380.07 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS7:SG1:CYS128:SG
2disulfidesing1:CYS65:SG1:CYS81:SG
3disulfidesing1:CYS77:SG1:CYS95:SG

Source organism
Gallus gallus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.9 %, Completeness: 84.4 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

Total1H
All84.4 % (639 of 757)84.4 % (639 of 757)
Backbone93.8 % (242 of 258)93.8 % (242 of 258)
Sidechain79.4 % (396 of 499)79.4 % (396 of 499)
Aromatic90.8 % (59 of 65)90.8 % (59 of 65)
Methyl95.1 % (58 of 61)95.1 % (58 of 61)

1. hen egg white lysozyme variant

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV AAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRAKGTD VQAWIRGCRL

Sample

Temperature 298 (±0.1) K, pH 3.8 (±0.1)


#NameIsotope labelingTypeConcentration
1hen egg white lysozyme variant[U-15N]0.2 ~ 1.0 mM

Release date
2005-04-03
Citation 1
NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins
Yokota, A., Hirai, K., Miyauchi, H., Iimura, S., Noda, Y., Inoue, K., Akasaka, K., Tachibana, H., Segawa, S.
Biochemistry (2004), 43, 6663-6669, PubMed 15157100 , DOI 10.1021/bi049967w ,
Citation 2
Characterisation of the dominant oxidative folding intermediate of hen lysozyme
J. Mol. Biol. (1999), 290, 781-796, PubMed 10395829 , DOI 10.1006/jmbi.1999.2915 ,
Citation 3
NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , DOI 10.1021/bi0113418 ,
Related entities 1. hen egg white lysozyme, : 1 : 103 : 99 entities Detail
Interaction partners 1. hen egg white lysozyme, : 8 interactors Detail
Keywords Lysozyme Variants, NMR, Protein Folding, Three-Disulfide Intermediate