BMRB: 644

Proline Assignments and Identification of the Cis K116/P117 Peptide Bond in Liganded Staphylococcal Nuclease Using Isotope Edited 2D NMR Spectroscopy

Authors

Torchia, D.A., Sparks, S.W., Young, P.E., Bax, A.

Assembly

micrococcal nuclease

Entity

1. micrococcal nuclease (polymer), 156 monomers, 17603.95 Da Detail

MDPTVYSATS TKKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

Formula weight

17603.95 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 5.8 %, Completeness: 4.7 %, Completeness (bb): 3.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	4.7 % (79 of 1698)	5.9 % (58 of 980)	2.9 % (21 of 718)
Backbone	3.5 % (27 of 773)	3.8 % (12 of 315)	3.3 % (15 of 458)
Sidechain	5.1 % (55 of 1071)	6.9 % (46 of 665)	2.2 % (9 of 406)
Aromatic	0.0 % (0 of 121)	0.0 % (0 of 61)	0.0 % (0 of 60)
Methyl	1.3 % (2 of 154)	1.3 % (1 of 77)	1.3 % (1 of 77)

1. micrococcal nuclease

MDPTVYSATS TKKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

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Sample

Temperature 310 K, pH 7.4

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Release date

1995-07-30

Citation

Influence of exertional hypoxemia on cerebral oxygenation in fibrotic interstitial lung disease
Torchia, D.A., Sparks, S.W., Young, P.E., Bax, A.
Respir. Physiol. Neurobiol. (2021), 285, 103601-103601, PubMed 33333239 , DOI 10.1016/j.resp.2020.103601 , Abstract

Related entities 1. micrococcal nuclease, : 1 : 129 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail