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BMRB: 6449


6449
8PP0
Backbone 1H, 15N and 13C chemical shift assignments for human retinoid X receptor ligand-binding domain with and without 9-cis retinoic acid
Authors
Lu, J., Cistola, D.P., Ellen, L.
Assembly
RXRalpha ligand-binding domain dimer
Entity
1. RXRalpha ligand-binding domain (polymer, Thiol state: all free), 240 monomers, 26822.72 × 2 Da Detail

TSSANEDMPV ERILEAELAV EPKTETYVEA NMGLNPSSPN DPVTNICQAA DKQLFTLVEW AKRIPHFSEL PLDDQVILLR AGWNELLIAS FSHRSIAVKD GILLATGLHV HRNSAHSAGV GAIFDRVLTE LVSKMRDMQM DKTELGCLRA IVLFNPDSKG LSNPAEVEAL REKVYASLEA YCKHKYPEQP GRFAKLLLRL PALRSIGLKC LEHLFFFKLI GDTPIDTFLM EMLEAPHQMT


2. REA (non-polymer), 300.435 Da
Total weight
53945.875 Da
Max. entity weight
26822.72 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 67.5 %, Completeness: 26.8 %, Completeness (bb): 47.8 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All26.8 % (753 of 2807)15.5 % (225 of 1456)34.9 % (387 of 1108)58.0 % (141 of 243)
Backbone47.8 % (675 of 1412)33.5 % (160 of 477)52.9 % (375 of 709)61.9 % (140 of 226)
Sidechain 9.4 % (153 of 1624) 6.7 % (66 of 979)13.9 % (87 of 628) 0.0 % (0 of 17)
Aromatic 6.9 % (13 of 188)13.8 % (13 of 94) 0.0 % (0 of 92) 0.0 % (0 of 2)
Methyl 9.9 % (30 of 302)11.3 % (17 of 151) 8.6 % (13 of 151)

1. RXRalpha ligand-binding domain

TSSANEDMPV ERILEAELAV EPKTETYVEA NMGLNPSSPN DPVTNICQAA DKQLFTLVEW AKRIPHFSEL PLDDQVILLR AGWNELLIAS FSHRSIAVKD GILLATGLHV HRNSAHSAGV GAIFDRVLTE LVSKMRDMQM DKTELGCLRA IVLFNPDSKG LSNPAEVEAL REKVYASLEA YCKHKYPEQP GRFAKLLLRL PALRSIGLKC LEHLFFFKLI GDTPIDTFLM EMLEAPHQMT

Show sample condition
Sample

Temperature 298 (±0.1) K, pH 7.4 (±0.2)


#NameIsotope labelingTypeConcentration
1RXRalpha ligand-binding domain[U-2H; U-13C; U-15N]1.0 mM
2RETINOIC ACID1.0 mM

LACS Plot; CA
Referencing offset: 0.41 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.41 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.07 ppm, Outliers: 1 Detail
Release date
2006-04-16
Citation 1
Analysis of ligand binding and protein dynamics of human retinoid X receptor alpha ligand-binding domain by nuclear magnetic resonance
Lu, J., Cistola, D.P., Li, E.
Biochemistry (2006), 45, 1629-1639, PubMed 16460010 , DOI 10.1021/bi051474j ,
Show more 3 citaions
Citation 2
Crystal structure of the ligand-binding domain of the human nuclear receptor RXR-alpha
Bourguet, W.
Nature (1995), 375, 377-382, PubMed 7760929 , DOI 10.1038/375377a0 ,
Citation 3
Crystal structure of the human RXRalpha ligand-binding domain bound to its natural ligand: 9-cis retinoic acid
Egea, P.F.
EMBO J. (2000), 19, 2592-2601, PubMed 10835357 , DOI 10.1093/emboj/19.11.2592 ,
Citation 4
Ligand-induced stabilization of PPARgamma monitored by NMR spectroscopy: implications for nuclear receptor activation
Johnson, B.A.
J. Mol. Biol. (2000), 298, 187-194, PubMed 10764590 , DOI 10.1006/jmbi.2000.3636 ,
Related entities 1. RXRalpha ligand-binding domain, : 1 : 36 : 54 : 150 entities Detail
More details for 241 related entities
Interaction partners 1. RXRalpha ligand-binding domain, : 53 interactors Detail
More details for 53 interactors identified by 35 citations
Experiments performed 8 experiments Detail
Chemical shift validation 4 contents Detail
Keywords NMR, X-ray structure