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Internal Dynamics of Human Ubiquitin Revealed by 13C-Relaxation Studies of Randomly Fractionally Labeled Protein
Authors
Wand, A.JOSHUA., Urbauer, J.L., McEvoy, R.P., Bieber, R.J.
Assembly
Ubiquitin
Entity
1. Ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG


Formula weight
8564.731 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, order_parameters
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 91.7 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.7 % (837 of 913)91.4 % (437 of 478)93.4 % (327 of 350)85.9 % (73 of 85)
Backbone98.9 % (439 of 444)100.0 % (149 of 149)97.7 % (217 of 222)100.0 % (73 of 73)
Sidechain86.6 % (467 of 539)87.5 % (288 of 329)90.4 % (179 of 198) 0.0 % (0 of 12)
Aromatic 0.0 % (0 of 32) 0.0 % (0 of 16) 0.0 % (0 of 16)
Methyl100.0 % (98 of 98)100.0 % (49 of 49)100.0 % (49 of 49)

1. Ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Sample #1

Temperature 303 (±0) K


#NameIsotope labelingTypeConcentration
1Ubiquitin[U-15N; U-13C]2.0 ~ 4.0 mM
2kPO450 mM
3D2O10 %
4H2O90 %
Sample #2

Temperature 303 (±0) K


#NameIsotope labelingTypeConcentration
5Ubiquitin[U-15N; U-13C]2.0 ~ 4.0 mM
6kPO450 mM
7D2O100 %

LACS Plot; CA
Referencing offset: -0.37 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.37 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.75 ppm, Outliers: 2 Detail
Heteronucl. T1
202 T1 values in 4 lists
Coherence Sz, Field strength (1H) 750 MHz, 500 MHz, Temperature 303 (±0) K Detail
Heteronucl. NOE
93 NOE values in 2 lists
Value type peak integral, Field strength (1H) 500 MHz, Temperature 303 (±0) K Detail
Order parameters
100 S2 values in 3 lists
Temperature 303 (±0) K Detail
Release date
2011-08-10
Citation
Internal dynamics of human ubiquitin revealed by 13C-relaxation studies of randomly fractionally labeled protein
Wand, A.JOSHUA., Urbauer, J.L., McEvoy, R.P., Bieber, R.J.
Biochemistry (1996), 35, 6116-6125, PubMed 8634254 , DOI 10.1021/bi9530144 ,
Related entities 1. Ubiquitin, : 1 : 200 : 5 : 25 : 139 entities Detail
Interaction partners 1. Ubiquitin, : 24 interactors Detail
Experiments performed 5 experiments Detail