BMRB: 6478

The N-domain of the transcription factor AbrB

Authors

Coles, M., Truffault, V., Lupas, A.N.

Assembly

Transition state regulatory protein abrB

Entity

1. Transition state regulatory protein abrB (polymer, Thiol state: not present), 53 monomers, 6146.267 × 2 Da Detail

MFMKSTGIVR KVDELGRVVI PIELRRTLGI AEKDALEIYV DDEKIILKKY KPN

Total weight

12292.534 Da

Max. entity weight

6146.267 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.0 %, Completeness (bb): 93.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.0 % (599 of 658)	93.9 % (326 of 347)	85.7 % (222 of 259)	98.1 % (51 of 52)
Backbone	93.3 % (293 of 314)	98.1 % (105 of 107)	88.5 % (138 of 156)	98.0 % (50 of 51)
Sidechain	89.6 % (353 of 394)	92.1 % (221 of 240)	85.6 % (131 of 153)	100.0 % (1 of 1)
Aromatic	46.2 % (12 of 26)	92.3 % (12 of 13)	0.0 % (0 of 13)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. Transition state regulatory protein abrB

MFMKSTGIVR KVDELGRVVI PIELRRTLGI AEKDALEIYV DDEKIILKKY KPN

Show sample condition

Sample

Temperature 305 (±0.1) K, pH 5.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Transition state regulatory protein abrB	[U-15N]		0.25 mM
6	Transition state regulatory protein abrB	[U-13C]		0.25 mM
7	KPO4			20.0 mM
8	KCl			50.0 mM
9	NaN3			0.02 %

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LACS Plot; CA

Referencing offset: 2.62 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.62 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 1YFB, Strand ID: A, B Detail

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Release date

2005-06-11

Citation

AbrB-like transcription factors assume a swapped hairpin fold that is evolutionarily related to double-psi beta barrels
Coles, M., Djuranovic, S., Soding, J., Frickey, T., Koretke, K., Truffault, V., Martin, J., Lupas, A.N.
Structure (2005), 13, 919-928, PubMed 15939023 , DOI 10.1016/j.str.2005.03.017 , Abstract

Related entities 1. Transition state regulatory protein abrB, : 1 : 1 : 4 : 8 entities Detail

Interaction partners 1. Transition state regulatory protein abrB, : 1 interactors Detail