BMRBj - BMREntryMaster

Found 1 Document (7.98 seconds)

BMRB: 6483

1YJR

The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease

Authors

Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.

Assembly

Copper-transporting ATPase 1(E.C.3.6.3.4)

Entity

1. Copper-transporting ATPase 1(E.C.3.6.3.4) (polymer, Thiol state: all free), 75 monomers, 8205.530 Da Detail

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEPS LVKIE

Formula weight

8205.53 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 81.6 %, Completeness (bb): 93.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	81.6 % (418 of 512)	79.7 % (350 of 439)	93.2 % (68 of 73)
Backbone	93.3 % (210 of 225)	93.5 % (143 of 153)	93.1 % (67 of 72)
Sidechain	72.5 % (208 of 287)	72.4 % (207 of 286)	100.0 % (1 of 1)
Aromatic	57.1 % (12 of 21)	57.1 % (12 of 21)
Methyl	98.1 % (51 of 52)	98.1 % (51 of 52)

1. Copper-transporting ATPase 1

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEPS LVKIE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1YJR, Strand ID: A Detail

Release date

2005-11-13

Citation

An atomic-level investigation of the disease-causing A629P mutant of the Menkes protein, ATP7A
Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.
J. Mol. Biol. (2005), 352, 409-417, PubMed 16083905 , DOI 10.1016/j.jmb.2005.07.034 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 6480 released on 2005-11-13
    Title The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease
  BMRB: 6481 released on 2005-11-13
    Title The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease
  BMRB: 6482 released on 2005-11-13
    Title The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease

Related entities 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 1 : 1 : 4 : 346 entities Detail

Interaction partners 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

2 CBCANH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

3 CBCACONH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

4 HNCO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

5 HNCACO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

6 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

7 2D TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

8 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

nullKeywords copper(I), metal homeostasis, metallochaperone, protein-protein interaction

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Found 1 Document (7.98 seconds)

BMRB: 6483

Sample

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 1 : 1 : 4 : 346 entities Detail

Interaction partners 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 5 interactors Detail

Experiments performed 8 experiments Detail

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