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BMRB: 6541

6O20

1H, 13C and 15N chemical shift assignments for stereo array isotope labeled (SAIL) calmodulin

Authors

Kainosho, M., Torizawa, T., Terauchi, T., Ono, A., Guntert, P.

Assembly

Ca-bound calmodulin

Entity

1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. CA (non-polymer), 40.078 × 4 Da

Total weight

16866.523 Da

Max. entity weight

16706.21 Da

Source organism

Xenopus laevis

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.1 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.1 % (1404 of 1670)	74.6 % (647 of 867)	92.9 % (599 of 645)	100.0 % (158 of 158)
Backbone	98.5 % (871 of 884)	96.4 % (294 of 305)	99.5 % (431 of 433)	100.0 % (146 of 146)
Sidechain	72.7 % (671 of 923)	62.8 % (353 of 562)	87.7 % (306 of 349)	100.0 % (12 of 12)
Aromatic	41.0 % (41 of 100)	50.0 % (25 of 50)	32.0 % (16 of 50)
Methyl	86.6 % (123 of 142)	85.9 % (61 of 71)	87.3 % (62 of 71)

1. calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

Show sample condition

Sample

Temperature 310 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	calmodulin	SAIL (stereo array isotope labeled)	0.7 mM
2	MES-d13		5 mM
3	bis-Tris-d19		10 mM
4	CaCl2		5 mM
5	NaN3		0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.4 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.4 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.58 ppm, Outliers: 1 Detail

Release date

2006-04-24

Citation 1

Optimal isotope labelling for NMR protein structure determinations
Kainosho, M., Torizawa, T., Iwashita, Y., Terauchi, T., Ono, A., Guntert, P.
Nature (2006), 440, 52-57, PubMed 16511487 , DOI 10.1038/nature04525 , Abstract

Show more 1 citaion

Citation 2

Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol
Torizawa, T., Shimizu, M., Taoka, M., Miyano, H., Kainosho, M.
J. Biomol. NMR (2004), 30, 311-325, PubMed 15754057 , DOI 10.1007/s10858-004-3534-2 , Abstract

Hide non-primary 1 citaion

Related entities 1. calmodulin, : 1 : 111 : 6 : 3 : 178 entities Detail

Interaction partners 1. calmodulin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 [1H,15N]-HSQC