BMRB: 6584

Backbone assignment of the E2 ubiquitin conjugating enzyme UbcH5alpha

Authors

Saxena, K., Jacobs, D.M., Vogtherr, M., Grimme, S., Elshorst, B., Fiebig, K.M., Schwalbe, H.

Assembly

E2

Entity

1. E2 (polymer, Thiol state: all free), 168 monomers, 19043.37 Da Detail

MGHHHHHHHH HHSSGHIEGR HMALKRIQKE LSDLQRDPPA HCSAGPVGDD LFHWQATIMG PPDSAYQGGV FKLTVHFPTD YPSKPPKIAF TTKIYHPNIN SNGSICLDIL RSQWSPALTV SKVLLSICSL LCDPNPDDPL VPDIAQIYKS DKEKYNRHAR EWTQKYAM

Formula weight

19043.37 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.3 %, Completeness: 34.3 %, Completeness (bb): 60.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	34.3 % (675 of 1967)	21.7 % (223 of 1026)	40.1 % (310 of 773)	84.5 % (142 of 168)
Backbone	60.0 % (587 of 978)	48.5 % (160 of 330)	58.0 % (287 of 495)	91.5 % (140 of 153)
Sidechain	19.0 % (218 of 1148)	9.1 % (63 of 696)	35.0 % (153 of 437)	13.3 % (2 of 15)
Aromatic	12.0 % (23 of 192)	12.5 % (12 of 96)	11.8 % (11 of 93)	0.0 % (0 of 3)
Methyl	11.0 % (17 of 154)	7.8 % (6 of 77)	14.3 % (11 of 77)

1. Ubiquitin ligating enzyme

MGHHHHHHHH HHSSGHIEGR HMALKRIQKE LSDLQRDPPA HCSAGPVGDD LFHWQATIMG PPDSAYQGGV FKLTVHFPTD YPSKPPKIAF TTKIYHPNIN SNGSICLDIL RSQWSPALTV SKVLLSICSL LCDPNPDDPL VPDIAQIYKS DKEKYNRHAR EWTQKYAM

Show sample condition

Sample

Temperature 295 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin ligating enzyme	[U-13C; U-15N; U-60% 2H]		1.7 mM
2	sodium sulphate			200 mM
3	sodium phosphate			50 mM
4	DTT			5 mM

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LACS Plot; CA

Referencing offset: 2.49 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
2H	DSS	methyl protons	0.0 ppm	null	indirect	0.1535061
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
31P	DSS	methyl protons	0.0 ppm	null	indirect	0.4048086

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LACS Plot; CB

Referencing offset: 2.49 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
2H	DSS	methyl protons	0.0 ppm	null	indirect	0.1535061
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
31P	DSS	methyl protons	0.0 ppm	null	indirect	0.4048086

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Release date

2005-10-24

Citation 1

Backbone NMR assignment of the C-terminal haemopexin-like domain (HPLD) of human matrix metalloproteinase MMP-13
Saxena, K., Jacobs, D.M., Vogtherr, M., Grimme, S., Elshorst, B., Pescatore, B., Betz, M., Schieborr, U., Langer, T., Schwalbe, H., Fiebig, K.
J. Biomol. NMR (2005), 32, 337-337, PubMed 16211490 , DOI 10.1007/s10858-005-0463-7 , Abstract

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Citation 2

Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53
Scheffner, M., Huibregtse, J.M., Howley, P.M.
Proc. Natl. Acad. Sci. U. S. A. (1994), 91, 8797-8801, PubMed 8090726 , DOI: , Abstract

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Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. E2, : 1 : 11 : 282 entities Detail

Interaction partners 1. E2, : 110 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail