BMRB: 6589

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chromo Domain of cpSRP43

Authors

Vaithiyalingam, S., Thallapuranam, S.KRISHNASWAMY., Philominathan, L.SAGAYA-THERESA., Henry, R., Yu, C.

Assembly

Chromo domain 1 of cpSRP43

Entity

1. Chromo domain 1 of cpSRP43 (polymer, Thiol state: not present), 47 monomers, 5044.476 Da Detail

GSGEVNKIIG SRTAGEGAME YLIEWKDGHS PSWVPSSYIA ADVVSEY

Formula weight

5044.476 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

Torsional angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 71.2 %, Completeness (bb): 86.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	71.2 % (364 of 511)	80.1 % (209 of 261)	54.5 % (110 of 202)	93.8 % (45 of 48)
Backbone	86.0 % (239 of 278)	89.8 % (88 of 98)	80.7 % (109 of 135)	93.3 % (42 of 45)
Sidechain	55.8 % (153 of 274)	71.8 % (117 of 163)	30.6 % (33 of 108)	100.0 % (3 of 3)
Aromatic	50.0 % (26 of 52)	92.3 % (24 of 26)	0.0 % (0 of 24)	100.0 % (2 of 2)
Methyl	50.0 % (23 of 46)	82.6 % (19 of 23)	17.4 % (4 of 23)

1. Chromo 1

GSGEVNKIIG SRTAGEGAME YLIEWKDGHS PSWVPSSYIA ADVVSEY

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD1 - Chromo domain 1	[U-15N; U-13C]		0.75 (±0.01) mM
2	phosphate buffer			100 mM
3	NaCl			100 mM

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LACS Plot; CA

Referencing offset: 2.69 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.69 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.24 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X32, Strand ID: A Detail

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Release date

2007-02-04

Citation

Three-dimensional solution structures of the chromodomains of cpSRP43
Sivaraja, V., Suresh Kumar, T.KRISHNASWAMY., Leena, P.SAGAYA-THERESA., Chang, A., Vidya, C., Goforth, R.L., Rajalingam, D., Arvind, K., Ye, J., Chou, J., Henry, R., Yu, C.
J. Biol. Chem. (2005), 280, 41465-41471, PubMed 16183644 , DOI 10.1074/jbc.M507077200 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. Chromo domain 1 of cpSRP43, : 1 : 5 : 38 entities Detail

Interaction partners 1. Chromo domain 1 of cpSRP43, : 21 interactors Detail

Experiments performed 7 experiments Detail