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BMRB: 6590

1YZS

1H, 13C, and 15N Chemical Shifts Assignments for Human Sulfiredoxin (srx)

Authors

Gruschus, J.M., Lee, D., Rhee, S., Ferretti, J.A.

Assembly

srx monomer

Entity

1. srx monomer (polymer, Thiol state: all free), 121 monomers, 12776.26 Da Detail

GAPEGPGPSG GAQGGSIHSG RIAAVHNVPL SVLIRPLPSV LDPAKVQSLV DTIREDPDSV PPIDVLWIKG AQGGDYFYSF GGCHRYAAYQ QLQRETIPAK LVQSTLSDLR VYLGASTPDL Q

Formula weight

12776.26 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.1 %, Completeness (bb): 83.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1206 of 1339)	96.5 % (669 of 693)	79.7 % (420 of 527)	98.3 % (117 of 119)
Backbone	83.9 % (589 of 702)	96.7 % (236 of 244)	70.5 % (246 of 349)	98.2 % (107 of 109)
Sidechain	96.6 % (719 of 744)	95.8 % (430 of 449)	98.2 % (280 of 285)	90.0 % (9 of 10)
Aromatic	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (41 of 41)	100.0 % (1 of 1)
Methyl	99.3 % (143 of 144)	98.6 % (71 of 72)	100.0 % (72 of 72)

1. sulfiredoxin

GAPEGPGPSG GAQGGSIHSG RIAAVHNVPL SVLIRPLPSV LDPAKVQSLV DTIREDPDSV PPIDVLWIKG AQGGDYFYSF GGCHRYAAYQ QLQRETIPAK LVQSTLSDLR VYLGASTPDL Q

Show sample condition

Sample #1

Temperature 300 (±.1) K, pH 7.3 (±.1)

#	Name	Isotope labeling	Concentration
1	sulfiredoxin	[U-15N]	0.7 (±0.1) mM
2	sodium phosphate		50 (±1.0) mM
3	NaCl		100 (±1.0) mM
4	DTT		2 (±0.1) mM

Sample #2

Temperature 300 (±.1) K, pH 7.3 (±.1)

#	Name	Isotope labeling	Concentration
5	sulfiredoxin	[U-15N; U-13C]	0.7 (±0.1) mM
6	sodium phosphate		50 (±1.0) mM
7	NaCl		100 (±1.0) mM
8	DTT		2 (±0.1) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: -0.4 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: -0.4 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Release date

2005-10-25

Citation

1H, 15N, and 13C chemical shift assignments of the human Sulfiredoxin (hSrx)
Lee, D., Rhee, S., Ferretti, J., Gruschus, J.M.
J. Biomol. NMR (2005), 32, 339-339, PubMed 16211494 , DOI 10.1007/s10858-005-0472-6 , Abstract

Related entities 1. srx monomer, : 1 : 3 : 6 : 9 entities Detail

Interaction partners 1. srx monomer, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 1H15N_HSQC