BMRB: 6612

NMR structure of unliagnded MDM2

Authors

Uhrinova, S., Uhrin, D., Powers, H., Watt, K., Zheleva, D., Fischer, P., McInnes, C., Barlow, P.N.

Assembly

Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-)

Entity

1. Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-) (polymer, Thiol state: all free), 119 monomers, 13556.47 Da Detail

MCNTNMSVPT DGAVTTSQIP ASEQETLVRP KPLLLKLLKS VGAQKDTYTM KEVLFYLGQY IMTKRLYDEK QQHIVYCSND LLGDLFGVPS FSVKEHRKIY TMIYRNLVVV NQQESSDSS

Formula weight

13556.47 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 72.8 %, Completeness (bb): 74.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	72.8 % (1032 of 1418)	77.9 % (575 of 738)	63.5 % (351 of 553)	83.5 % (106 of 127)
Backbone	74.0 % (521 of 704)	87.4 % (208 of 238)	59.4 % (209 of 352)	91.2 % (104 of 114)
Sidechain	73.3 % (607 of 828)	73.6 % (368 of 500)	75.2 % (237 of 315)	15.4 % (2 of 13)
Aromatic	48.9 % (46 of 94)	51.1 % (24 of 47)	46.8 % (22 of 47)
Methyl	94.3 % (132 of 140)	97.1 % (68 of 70)	91.4 % (64 of 70)

1. Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-)

MCNTNMSVPT DGAVTTSQIP ASEQETLVRP KPLLLKLLKS VGAQKDTYTM KEVLFYLGQY IMTKRLYDEK QQHIVYCSND LLGDLFGVPS FSVKEHRKIY TMIYRNLVVV NQQESSDSS

Show sample condition

Sample

Temperature 288 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-)	[U-13C; U-15N]		0.5 mM
2	sodium acetate	[U-2H]		60 mM
3	phosphate buffer			60 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: 2.52 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.87 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.52 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.87 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.87 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 24 models in PDB: 1Z1M, Strand ID: A Detail

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Release date

2005-10-26

Citation

Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding
Uhrinova, S., Uhrin, D., Powers, H., Watt, K., Zheleva, D., Fischer, P., McInnes, C., Barlow, P.N.
J. Mol. Biol. (2005), 350, 587-598, PubMed 15953616 , DOI 10.1016/j.jmb.2005.05.010 , Abstract

Related entities 1. Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-), : 1 : 38 : 61 entities Detail

Interaction partners 1. Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-), : 170 interactors Detail