BMRBj - BMREntryMaster

Found 1 Document (1.295 seconds)

BMRB: 6679

1ZXH

G311

Authors

He, Y., Yeh, C., Alexander, P., Bryan, P., Orban, J.

Assembly

G311

Entity

1. G311 (polymer, Thiol state: not present), 56 monomers, 6297.917 Da Detail

MYYLVVNKGQ NAFYETLTKA VDAETARNAF IQSLKDDGVQ GVWTYDDATK TFTVQA

Formula weight

6297.917 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.0 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.0 % (574 of 645)	87.8 % (287 of 327)	89.4 % (227 of 254)	93.8 % (60 of 64)
Backbone	95.5 % (321 of 336)	98.3 % (113 of 115)	93.3 % (154 of 165)	96.4 % (54 of 56)
Sidechain	83.4 % (302 of 362)	82.1 % (174 of 212)	85.9 % (122 of 142)	75.0 % (6 of 8)
Aromatic	97.3 % (72 of 74)	100.0 % (37 of 37)	94.4 % (34 of 36)	100.0 % (1 of 1)
Methyl	92.6 % (63 of 68)	97.1 % (33 of 34)	88.2 % (30 of 34)

1. G311

MYYLVVNKGQ NAFYETLTKA VDAETARNAF IQSLKDDGVQ GVWTYDDATK TFTVQA

Show sample condition

Sample

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 3.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 3.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -3.18 ppm, Outliers: 1 Detail

Release date

2005-11-02

Citation

Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds
He, Y., Yeh, D., Alexander, P., Bryan, P., Orban, J.
Biochemistry (2005), 44, 14055-14061, PubMed 16245921 , DOI 10.1021/bi051232j , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 6680 released on 2005-11-02
Title Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for A219.

Related entities 1. G311, : 1 : 122 entities Detail

Experiments performed 10 experiments Detail

1 3D HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

2 3D HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

3 3D CBCACONH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

4 3D HBHA(CBCACO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

5 3D(H)C(CO)NH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

6 3d h(CCO)NH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

7 2D TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

8 2D CBHD

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

9 2D CBHE

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

10 2D TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	G311	[U-13C; U-15N]	0.2 ~ 0.4 mM
2	postassium phosphate buffer		100 mM
3	D2O		10 %

Sample #2

Temperature 275 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
4	G311	[U-15N]	0.2 ~ 0.4 mM
5	postassium phosphate buffer		100 mM
6	D2O		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6679_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6679_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	641		100.0 (chain: 1, length: 56)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 56, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 355
  - ¹³C chemical shifts: 226
  - ¹⁵N chemical shifts: 60
- Completeness of assignments
  - Entity_assemble_ID: 1
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	327	287	87.8
¹³C chemical shifts	254	226	89.0
¹⁵N chemical shifts	65	60	92.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	115	113	98.3
¹³C chemical shifts	112	104	92.9
¹⁵N chemical shifts	56	54	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	212	174	82.1
¹³C chemical shifts	142	122	85.9
¹⁵N chemical shifts	9	6	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	35	34	97.1
¹³C chemical shifts	35	31	88.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	37	37	100.0
¹³C chemical shifts	36	34	94.4
¹⁵N chemical shifts	1	1	100.0

Keywords IgG-binding, NMR, phage display, protein G, iGg-BINDING, nmr, PHAGE DISPLAY, PROTEIN g

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Found 1 Document (1.295 seconds)

BMRB: 6679

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. G311, : 1 : 122 entities Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail