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G83N TPR domain of Ppp5
Authors
Cliff, M.J., Harris, R., Barford, D., Ladbury, J.E., Williams, M.A.
Assembly
PP5-TPR-peptide complex
Entity
1. tetratricopeptide domain from Ppp5 (polymer, Thiol state: all free), 140 monomers, 16179.12 Da Detail

MSGHHHHHHT DPPADGALKR AEELKTQAND YFKAKDYENA IKFYSQAIEL NPSNAIYYGN RSLAYLRTEC YGYALNDATR AIELDKKYIK GYYRRAASNM ALGKFRAALR DYETVVKVKP HDKDAKMKYQ ECNKIVKQKA


2. tetratricopeptide domain from Ppp5 (polymer, Thiol state: not present), 6 monomers, 647.6946 Da Detail

XMEEVD


Total weight
16826.814 Da
Max. entity weight
16179.12 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
ARIA
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.2 %, Completeness: 86.7 %, Completeness (bb): 87.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.7 % (1502 of 1732)88.4 % (799 of 904)84.6 % (564 of 667)86.3 % (139 of 161)
Backbone87.4 % (748 of 856)90.9 % (260 of 286)84.4 % (362 of 429)89.4 % (126 of 141)
Sidechain86.4 % (877 of 1015)87.2 % (539 of 618)86.2 % (325 of 377)65.0 % (13 of 20)
Aromatic82.1 % (133 of 162)82.7 % (67 of 81)81.5 % (66 of 81)
Methyl98.4 % (126 of 128)100.0 % (64 of 64)96.9 % (62 of 64)

1. tetratricopeptide domain from Ppp5

MSGHHHHHHT DPPADGALKR AEELKTQAND YFKAKDYENA IKFYSQAIEL NPSNAIYYGN RSLAYLRTEC YGYALNDATR AIELDKKYIK GYYRRAASNM ALGKFRAALR DYETVVKVKP HDKDAKMKYQ ECNKIVKQKA

2. tetratricopeptide domain from Ppp5

XMEEVD

Sample

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions


#NameIsotope labelingTypeConcentration
1tetratricopeptide domain from Ppp5[U-13C; U-15N]protein0.75 (±0.4) mM
2Hsp90 pentapeptideligand0.75 (±0.4) mM
3MESbuffer50 (±0.2) mM
4DTTreducing agent5 (±0.1) mM

Protein Blocks Logo
Calculated from 19 models in PDB: 2BUG, Strand ID: A, B Detail


Release date
2006-04-04
Citation
Conformational diversity in the TPR domain-mediated interaction of protein phosphatase 5 with Hsp90
Cliff, M.J., Harris, R., Barford, D., Ladbury, J.E., Williams, M.A.
Structure (2006), 14, 415-426, PubMed 16531226 , DOI 10.1016/j.str.2005.12.009 ,
Entries sharing articles Swiss-Prot: 2 entries Detail
  Swiss-Prot: P53041 released on 1996-10-01
    Title PPP5_HUMAN Entity Serine/threonine-protein phosphatase 5
  Swiss-Prot: P07900 released on 1988-08-01
    Title HS90A_HUMAN Entity Heat shock protein HSP 90-alpha
Experiments performed 10 experiments Detail
Keywords NMR assigment