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Found 1 Document (1.388 seconds)

BMRB: 6742

2AGA

De-ubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain

Authors

Mao, Y., Hodsdon, M.E., De Camilli, P.

Assembly

ataxin-3 josephin domain

Entity

1. ataxin-3 josephin domain (polymer, Thiol state: all free), 190 monomers, 21876.58 Da Detail

MPLGSMESIF HEKQEGSLCA QHCLNNLLQG EYFSPVELSS IAHQLDEEER MRMAEGGVTS EDYRTFLQQP SGNMDDSGFF SIQVISNALK VWGLELILFN SPEYQRLRID PINERSFICN YKEHWFTVRK LGKQWFNLNS LLTGPELISD TYLALFLAQL QQEGYSIFVV KGDLPDCEAD QLLQMIRVQQ

Formula weight

21876.58 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 89.9 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.9 % (2055 of 2286)	91.9 % (1096 of 1193)	86.3 % (761 of 882)	93.8 % (198 of 211)
Backbone	95.6 % (1076 of 1126)	96.6 % (372 of 385)	94.6 % (528 of 558)	96.2 % (176 of 183)
Sidechain	84.5 % (1131 of 1338)	89.0 % (719 of 808)	77.9 % (391 of 502)	75.0 % (21 of 28)
Aromatic	61.0 % (128 of 210)	81.0 % (85 of 105)	39.2 % (40 of 102)	100.0 % (3 of 3)
Methyl	95.0 % (190 of 200)	96.0 % (96 of 100)	94.0 % (94 of 100)

1. ataxin-3 josephin domain

Show sample condition

Sample

Temperature 298 (±2) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ataxin-3 josephin domain	[U-100% 13C; U-100% 15N]		1.5 mM
2	potassium phospate buffer			20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AGA, Strand ID: A Detail

Release date

2005-09-07

Citation

Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain
Mao, Y., Senic-Matuglia, F., Di Fiore, P.P., Polo, S., Hodsdon, M.E., De Camilli, P.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 12700-12705, PubMed 16118278 , DOI 10.1073/pnas.0506344102 , Abstract

Related entities 1. ataxin-3 josephin domain, : 1 : 5 : 35 entities Detail

Interaction partners 1. ataxin-3 josephin domain, : 30 interactors Detail

More details for 30 interactors identified by 14 citations

Experiments performed 10 experiments Detail

1 3D-HNCO