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BMRB: 6766

2AKL

Complete assignment of a hypothetical protein pa0128 form Pseudomonas aeruginosa. Northeast Structural Genomics Consortium Target PaT1.

Authors

Srisailam, S., Yee, A., Lukin, J., Lemak, A., Bansal, S., James, P., Arrowsmith, C.

Assembly

pa0128

Entity

1. phn-a like hypothetical protein (polymer, Thiol state: all free), 138 monomers, 14968.71 Da Detail

MGSSHHHHHH SSGRENLYFQ GHMVSTLPPC PQCNSEYTYE DGALLVCPEC AHEWSPNEAA TASDDGKVIK DSVGNVLQDG DTITVIKDLK VKGSSLVVKV GTKVKNIRLV DGDHDIDCKI DGIGAMKLKS EFVRKVGS

2. ZN (non-polymer), 65.409 Da

Total weight

15034.119 Da

Max. entity weight

14968.71 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Refine. method

Simulated Annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.4 %, Completeness: 84.9 %, Completeness (bb): 86.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (1295 of 1526)	84.5 % (666 of 788)	85.1 % (507 of 596)	85.9 % (122 of 142)
Backbone	86.9 % (711 of 818)	86.6 % (246 of 284)	87.3 % (350 of 401)	86.5 % (115 of 133)
Sidechain	83.4 % (695 of 833)	83.3 % (420 of 504)	83.7 % (268 of 320)	77.8 % (7 of 9)
Aromatic	40.2 % (37 of 92)	41.3 % (19 of 46)	37.8 % (17 of 45)	100.0 % (1 of 1)
Methyl	98.6 % (142 of 144)	98.6 % (71 of 72)	98.6 % (71 of 72)

1. phn-a like hypothetical protein

MGSSHHHHHH SSGRENLYFQ GHMVSTLPPC PQCNSEYTYE DGALLVCPEC AHEWSPNEAA TASDDGKVIK DSVGNVLQDG DTITVIKDLK VKGSSLVVKV GTKVKNIRLV DGDHDIDCKI DGIGAMKLKS EFVRKVGS

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical protein pa0128	[U-15N; U-13C]	protein	1 (±0.2) mM
2	sodium phosphates		buffer	20 (±0.2) mM
3	NaCl		salt	400 (±0.2) mM
4	DTT		reducing agent	15 (±0.2) mM
5	Benzamidine		additive	1 (±0.2) mM
6	Sodium Azide		microbial agent	0.01 (±0.01) %
7	Zinc Sulfate		ligand	20 (±0.1) uM
8	D2O		solvent	5 (±0.1) %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AKL, Strand ID: A Detail

Release date

2006-04-23

Citation

Sequence specific resonance assignment of a hypothetical protein PA0128 from Pseudomonas aeruginosa
Srisailam, S., Lukin, J.A., Lemak, A., Yee, A., Arrowsmith, C.H.
J. Biomol. NMR (2006), 36 Suppl 1, 27-27, PubMed 16703420 , DOI 10.1007/s10858-006-0011-0 , Abstract

Related entities 1. phn-a like hypothetical protein, : 1 : 4 entities Detail

Experiments performed 15 experiments Detail

1 HNCO