BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	99.0 % (767 of 775)	99.3 % (397 of 400)	98.4 % (301 of 306)	100.0 % (69 of 69)
Backbone	98.5 % (384 of 390)	99.2 % (130 of 131)	97.4 % (191 of 196)	100.0 % (63 of 63)
Sidechain	99.6 % (447 of 449)	99.3 % (267 of 269)	100.0 % (174 of 174)	100.0 % (6 of 6)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (35 of 35)	100.0 % (3 of 3)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. YFHJ

MGLKWTDSRE IGEALYDAYP DLDPKTVRFT DMHQWICDLE DFDDDPQASN EKILEAILLV WLDEAE

Show sample condition

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2BZT, Strand ID: A Detail

Release date

2006-06-26

Citation

YfhJ, a molecular adaptor in iron-sulfur cluster formation or a frataxin-like protein?
Pastore, C., Adinolfi, S., Huynen, M.A., Rybin, V., Martin, S., Mayer, M., Bukau, B., Pastore, A.
Structure (2006), 14, 857-867, PubMed 16698547 , DOI 10.1016/j.str.2006.02.010 , Abstract

Related entities 1. YFHJ, : 1 : 2 : 1 : 9 entities Detail

Interaction partners 1. YFHJ, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 1H_13C_NOESY-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

2 1H_15N_NOESY-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

3 1H15N_TOCSY-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

4 1H15N_HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

5 1H13C_HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

6 HCCH_TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

7 CBCANH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

8 CBCACONH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

9 HNCA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

10 HNCOCA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

11 HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

12 HNHA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY-plus (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniformly labeling with 15N (U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
1	YFHJ	[U-98% 15N]	protein	0.5 mM
2	phosphate		buffer	10 mM
3	NaCl		salt	50 %
4	H20			90 %
5	D2O			10 %

Sample #2

Temperature 303 (±1) K, pH 6.5 (±0.1), Details Uniform labeling with 13C and 15N (U-99% 13C, U-98% 15N)

#	Name	Isotope labeling	Type	Concentration
6	YFHJ	[U-99% 13C; U-98 15N]	protein	0.6 mM
7	phosphate		buffer	10 mM
8	NaCl		salt	50 mM
9	H2O			90 %
10	D2O			10 %

nullKeywords iron sulphur clusters, ISC operon, NMR

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.787 seconds)

BMRB: 6776

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. YFHJ, : 1 : 2 : 1 : 9 entities Detail

Interaction partners 1. YFHJ, : 2 interactors Detail

Experiments performed 12 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2