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1H, 13C, and 15N chemical shift assignments for stereo-array isotope labelled (SAIL) maltodextrin-binding protein (MBP)
Authors
Kainosho, M., Torizawa, T., Iwashita, Y., Terauchi, T., Ono, A.MEI., Guntert, P.
Assembly
Maltodextrin-binding protein (MBP)
Entity
1. Maltodextrin-binding protein (polymer, Thiol state: not present), 370 monomers, 40706.77 Da Detail

KIEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPW AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK


2. BCD (non-polymer), 1134.984 Da
Total weight
41841.754 Da
Max. entity weight
40706.77 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.8 %, Completeness: 73.1 %, Completeness (bb): 82.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All73.1 % (3163 of 4327)72.9 % (1632 of 2240)68.9 % (1168 of 1694)92.4 % (363 of 393)
Backbone82.7 % (1777 of 2149)93.7 % (674 of 719)70.6 % (763 of 1081)97.4 % (340 of 349)
Sidechain67.6 % (1704 of 2519)63.0 % (958 of 1521)75.8 % (723 of 954)52.3 % (23 of 44)
Aromatic34.9 % (132 of 378)41.3 % (78 of 189)27.1 % (49 of 181)62.5 % (5 of 8)
Methyl81.4 % (342 of 420)82.4 % (173 of 210)80.5 % (169 of 210)

1. Maltodextrin-binding protein

KIEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPW AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK

Sample

Temperature 310 (±1) K, pH 7.2 (±0.1)


#NameIsotope labelingTypeConcentration
1Maltodextrin-binding protein0.9 mM
2beta-cyclodextrin3.3 mM
3sodium phosphate20 mM
4NaN33 mM
5CompleteMini protease inhibitor mix

LACS Plot; CA
Referencing offset: 0.0 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.0 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
Release date
2006-04-24
Citation
Optimal isotope labelling for NMR protein structure determinations
Kainosho, M., Torizawa, T., Iwashita, Y., Terauchi, T., Mei Ono, A., Guntert, P.
Nature (2006), 440, 52-57, PubMed 16511487 , DOI 10.1038/nature04525 ,
Related entities 1. Maltodextrin-binding protein, : 1 : 25 : 1 : 72 : 11 entities Detail
Interaction partners 1. Maltodextrin-binding protein, : 1 interactors Detail
Experiments performed 1 experiments Detail