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Solution structure of the C-terminal RpoN domain of aquifex aeolicus SIGMA-54 shows similarity to SIGMA-70
Authors
Doucleff, M., Malak, L.T., Pelton, J.G., Wemmer, D.E.
Assembly
RNA polymerase sigma factor RpoN
Entity
1. RNA polymerase sigma factor RpoN (polymer, Thiol state: not present), 76 monomers, 8949.237 Da Detail

TYSLRTFFVR ESAEGLTQGE LMKLIKEIVE NEDKRKPYSD QEIANILKEK GFKVARRTVA KYREMLGIPS SRERRI


Formula weight
8949.237 Da
Source organism
Aquifex aeolicus
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 86.8 %, Completeness: 67.8 %, Completeness (bb): 70.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All67.8 % (643 of 948)72.4 % (362 of 500)59.3 % (214 of 361)77.0 % (67 of 87)
Backbone70.1 % (314 of 448)84.0 % (126 of 150)55.8 % (125 of 224)85.1 % (63 of 74)
Sidechain68.2 % (390 of 572)67.4 % (236 of 350)71.8 % (150 of 209)30.8 % (4 of 13)
Aromatic40.7 % (22 of 54)81.5 % (22 of 27) 0.0 % (0 of 27)
Methyl85.0 % (68 of 80)85.0 % (34 of 40)85.0 % (34 of 40)

1. RNA polymerase sigma factor RpoN

TYSLRTFFVR ESAEGLTQGE LMKLIKEIVE NEDKRKPYSD QEIANILKEK GFKVARRTVA KYREMLGIPS SRERRI

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
1RNA polymerase sigma factor RpoN[U-15N]0.8 mM
2Hepes50 mM
3NaCl250 mM
4EDTA1 mM
5H2090 %
6D2010 %
Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
7RNA polymerase sigma factor RpoN[U-13C; U-15N]0.8 mM
8Hepes50 mM
9NaCl250 mM
10EDTA1 mM
11H2090 %
12D2010 %
Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
13RNA polymerase sigma factor RpoN[U-15N]0.8 mM
14Hepes50 mM
15NaCl250 mM
16EDTA1 mM
17H205 %
18D2095 %
Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
19RNA polymerase sigma factor RpoN[U-13C; U-15N]0.8 mM
20Hepes50 mM
21NaCl250 mM
22EDTA1 mM
23H2090 %
24D2010 %

LACS Plot; CA
Referencing offset: -0.34 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.34 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2AHQ, Strand ID: A Detail


Release date
2007-01-28
Citation
The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70
Doucleff, M., Malak, L.T., Pelton, J.G., Wemmer, D.E.
J. Biol. Chem. (2005), 280, 41530-41536, PubMed 16210314 , DOI 10.1074/jbc.M509010200 ,
Related entities 1. RNA polymerase sigma factor RpoN, : 1 : 1 : 2 : 24 entities Detail
Experiments performed 6 experiments Detail
Keywords RNA polymerase, Sigma-54, sigma factors, solution structure, transcription