BMRBj - BMREntryMaster

Found 1 Document (1.599 seconds)

BMRB: 6822

2L7H

The Structure of the Hamp Domain Implies a Rotational Mechanism in Transmembrane Signalling

Authors

Coles, M., Truffault, V., Hulko, M., Martin, J., Lupas, A.N.

Assembly

hypothetical protein AF1503

Entity

1. hypothetical protein AF1503 (polymer, Thiol state: not present), 56 monomers, 6142.907 × 2 Da Detail

GSSTITRPII ELSNTADKIA EGNLEAEVPH QNRADEIGIL AKSIERLRRS LKVAME

Total weight

12285.814 Da

Max. entity weight

6142.907 Da

Source organism

Archaeoglobus fulgidus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 94.3 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.3 % (596 of 632)	94.3 % (312 of 331)	94.7 % (230 of 243)	93.1 % (54 of 58)
Backbone	92.5 % (307 of 332)	92.0 % (104 of 113)	92.7 % (153 of 165)	92.6 % (50 of 54)
Sidechain	96.6 % (341 of 353)	95.4 % (208 of 218)	98.5 % (129 of 131)	100.0 % (4 of 4)
Aromatic	50.0 % (2 of 4)	50.0 % (1 of 2)	50.0 % (1 of 2)
Methyl	98.6 % (73 of 74)	97.3 % (36 of 37)	100.0 % (37 of 37)

1. hypothetical protein AF1503

GSSTITRPII ELSNTADKIA EGNLEAEVPH QNRADEIGIL AKSIERLRRS LKVAME

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Protein Blocks Logo

Calculated from 18 models in PDB: 2L7H, Strand ID: A, B Detail

Release date

2006-10-18

Citation

The HAMP domain structure implies helix rotation in transmembrane signaling
Hulko, M., Berndt, F., Gruber, M., Linder, J.U., Truffault, V., Schultz, A., Martin, J., Schultz, J.E., Lupas, A.N., Coles, M.
Cell (2006), 126, 929-940, PubMed 16959572 , DOI 10.1016/j.cell.2006.06.058 , Abstract

Related entities 1. hypothetical protein AF1503, : 1 : 12 : 52 entities Detail

Experiments performed 6 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker DMX (900 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	hypothetical protein AF1503	[U-15N]	1.0 mM
7	phosphate buffer		20 mM
8	NACL		150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
11	hypothetical protein AF1503	[U-13C; U-15N]	1.0 mM
12	phosphate buffer		20 mM
13	NACL		150 mM
14	H2O		90 %
15	D2O		10 %

2 2D NOESY

Instrument

Model Bruker DMX (900 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	hypothetical protein AF1503	[U-15N]	1.0 mM
7	phosphate buffer		20 mM
8	NACL		150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
11	hypothetical protein AF1503	[U-13C; U-15N]	1.0 mM
12	phosphate buffer		20 mM
13	NACL		150 mM
14	H2O		90 %
15	D2O		10 %

3 HNHA

Instrument

Model Bruker DMX (900 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	hypothetical protein AF1503	[U-15N]	1.0 mM
7	phosphate buffer		20 mM
8	NACL		150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
11	hypothetical protein AF1503	[U-13C; U-15N]	1.0 mM
12	phosphate buffer		20 mM
13	NACL		150 mM
14	H2O		90 %
15	D2O		10 %

4 2D 12C-FILTERED/13C-EDITED NOESY

Instrument

Model Bruker DMX (900 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	hypothetical protein AF1503	[U-15N]	1.0 mM
7	phosphate buffer		20 mM
8	NACL		150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
11	hypothetical protein AF1503	[U-13C; U-15N]	1.0 mM
12	phosphate buffer		20 mM
13	NACL		150 mM
14	H2O		90 %
15	D2O		10 %

5 3D-NNH-NOESY

Instrument

Model Bruker DMX (900 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	hypothetical protein AF1503	[U-15N]	1.0 mM
7	phosphate buffer		20 mM
8	NACL		150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
11	hypothetical protein AF1503	[U-13C; U-15N]	1.0 mM
12	phosphate buffer		20 mM
13	NACL		150 mM
14	H2O		90 %
15	D2O		10 %

6 3D-CNH-NOESY

Instrument

Model Bruker DMX (900 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Concentration
1	hypothetical protein AF1503	1.0 mM
2	phosphate buffer	20 mM
3	NACL	150 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	hypothetical protein AF1503	[U-15N]	1.0 mM
7	phosphate buffer		20 mM
8	NACL		150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
11	hypothetical protein AF1503	[U-13C; U-15N]	1.0 mM
12	phosphate buffer		20 mM
13	NACL		150 mM
14	H2O		90 %
15	D2O		10 %

nullKeywords COMPLEMENTARY X-DA PACKING, HOMODIMER, PARALLEL COILED-COIL

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Found 1 Document (1.599 seconds)

BMRB: 6822

Sample

Related entities 1. hypothetical protein AF1503, : 1 : 12 : 52 entities Detail

Experiments performed 6 experiments Detail

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