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Chemical Shift Assignments for V66W110 fragment of Staphylococcal Nuclease
Authors
Liu, D., Xie, T., Feng, Y., Shan, L., Ye, K., Wang, J.
Assembly
V66W110 fragment of staphylococcal nuclease
Entity
1. V66W110 fragment of staphylococcal nuclease (polymer, Thiol state: not present), 110 monomers, 12388.28 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK


Formula weight
12388.28 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Refine. method
simulated annealing, torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 89.1 %, Completeness: 64.2 %, Completeness (bb): 86.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All64.2 % (522 of 813)61.7 % (431 of 698)79.1 % (91 of 115)
Backbone86.3 % (276 of 320)86.5 % (186 of 215)85.7 % (90 of 105)
Sidechain49.9 % (246 of 493)50.7 % (245 of 483)10.0 % (1 of 10)
Aromatic60.9 % (28 of 46)60.0 % (27 of 45)100.0 % (1 of 1)
Methyl43.9 % (25 of 57)43.9 % (25 of 57)

1. V66W110 fragment of staphylococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK

Sample

Temperature 298 (±0.1) K, pH 4.9 (±0.1)


#NameIsotope labelingTypeConcentration
1V66W110 fragment of staphylococcal nuclease[U-95% 15N]1.0 ~ 2.5 mM
2deuterated acetate[U-99% 2H]50 (±1.0) mM

Protein Blocks Logo
Calculated from 12 models in PDB: 2F3V, Strand ID: A Detail


Release date
2007-07-09
Citation
Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease
Xie, T., Liu, D., Feng, Y., Shan, L., Wang, J.
Biophys. J. (2007), 92, 2090-2107, PubMed 17172296 , DOI 10.1529/biophysj.106.092155 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 6908 released on 2007-07-09
    Title 1H, 13C, and 15N Chemical Shift Assignments for SNase110 fragment of Staphylococcal Nuclease in 2M TMAO
  BMRB: 5536 released on 2003-12-07
    Title 1H, 13C, and 15N Chemical Shift Assignments for G88W110 fragment of Staphylococcal Nuclease
Related entities 1. V66W110 fragment of staphylococcal nuclease, : 1 : 2 : 74 : 38 entities Detail
Experiments performed 3 experiments Detail