BMRB: 6907

Chemical Shift Assignments for V66W110 fragment of Staphylococcal Nuclease

Authors

Liu, D., Xie, T., Feng, Y., Shan, L., Ye, K., Wang, J.

Assembly

V66W110 fragment of staphylococcal nuclease

Entity

1. V66W110 fragment of staphylococcal nuclease (polymer, Thiol state: not present), 110 monomers, 12388.28 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK

Formula weight

12388.28 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 65.6 %, Completeness (bb): 86.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	65.6 % (531 of 809)	63.0 % (440 of 698)	82.0 % (91 of 111)
Backbone	86.9 % (286 of 329)	87.5 % (196 of 224)	85.7 % (90 of 105)
Sidechain	51.0 % (245 of 480)	51.5 % (244 of 474)	16.7 % (1 of 6)
Aromatic	60.9 % (28 of 46)	60.0 % (27 of 45)	100.0 % (1 of 1)
Methyl	49.1 % (28 of 57)	49.1 % (28 of 57)

1. V66W110 fragment of staphylococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK

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Sample

Temperature 298 (±0.1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	V66W110 fragment of staphylococcal nuclease	[U-95% 15N]		1.0 ~ 2.5 mM
2	deuterated acetate	[U-99% 2H]		50 (±1.0) mM

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Protein Blocks Logo

Calculated from 12 models in PDB: 2F3V, Strand ID: A Detail

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Release date

2007-07-09

Citation

Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease
Xie, T., Liu, D., Feng, Y., Shan, L., Wang, J.
Biophys. J. (2007), 92, 2090-2107, PubMed 17172296 , DOI 10.1529/biophysj.106.092155 , Abstract

Related entities 1. V66W110 fragment of staphylococcal nuclease, : 1 : 2 : 75 : 41 entities Detail

Experiments performed 3 experiments Detail