BMRB: 6908

1H, 13C, and 15N Chemical Shift Assignments for SNase110 fragment of Staphylococcal Nuclease in 2M TMAO

Authors

Liu, D., Xie, T., Feng, Y., Shan, L., Ye, K., Wang, J.

Assembly

SNase110 fragment of Staphylococcal Nuclease

Entity

1. SNase110 fragment of Staphylococcal Nuclease (polymer, Thiol state: not present), 110 monomers, 12301.20 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK

Formula weight

12301.2 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 45.9 %, Completeness (bb): 54.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	45.9 % (604 of 1316)	65.2 % (452 of 693)	10.9 % (56 of 513)	87.3 % (96 of 110)
Backbone	54.0 % (351 of 650)	92.9 % (208 of 224)	14.6 % (47 of 321)	91.4 % (96 of 105)
Sidechain	34.2 % (262 of 767)	52.0 % (244 of 469)	6.1 % (18 of 293)	0.0 % (0 of 5)
Aromatic	29.5 % (23 of 78)	59.0 % (23 of 39)	0.0 % (0 of 39)
Methyl	36.4 % (43 of 118)	69.5 % (41 of 59)	3.4 % (2 of 59)

1. SNase110 fragment of Staphylococcal Nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK

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Sample

Temperature 298 (±0.1) K, pH 6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNase110 fragment of Staphylococcal Nuclease	[U-95% 13C; U-90% 15N]		1 (±0.1) mM
2	TMAO			2 (±0.1) M

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Protein Blocks Logo

Calculated from 12 models in PDB: 2F3W, Strand ID: A Detail

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Release date

2007-07-09

Citation

Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease
Xie, T., Liu, D., Feng, Y., Shan, L., Wang, J.
Biophys. J. (2007), 92, 2090-2107, PubMed 17172296 , DOI 10.1529/biophysj.106.092155 , Abstract

Related entities 1. SNase110 fragment of Staphylococcal Nuclease, : 1 : 40 : 28 : 36 : 13 entities Detail

Experiments performed 4 experiments Detail