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Found 1 Document (1.571 seconds)

BMRB: 6945

2FS1

solution structure of PSD-1

Authors

He, Y., Rozak, D., Sari, N., Chen, Y., Bryan, P., Orban, J.

Assembly

PSD-1

Entity

1. PSD-1 (polymer, Thiol state: not present), 56 monomers, 6137.956 Da Detail

MEAVDANSLA QAKEAAIKEL KQYGIGDYYI KLINNAKTVE GVESLKNEIL KALPTE

Formula weight

6137.956 Da

Exptl. method

NMR

Refine. method

Simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.2 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.2 % (575 of 652)	89.4 % (303 of 339)	84.1 % (212 of 252)	98.4 % (60 of 61)
Backbone	96.7 % (323 of 334)	95.6 % (109 of 114)	97.0 % (160 of 165)	98.2 % (54 of 55)
Sidechain	81.9 % (304 of 371)	86.2 % (194 of 225)	74.3 % (104 of 140)	100.0 % (6 of 6)
Aromatic	50.0 % (12 of 24)	100.0 % (12 of 12)	0.0 % (0 of 12)
Methyl	77.6 % (59 of 76)	81.6 % (31 of 38)	73.7 % (28 of 38)

1. PSD-1

MEAVDANSLA QAKEAAIKEL KQYGIGDYYI KLINNAKTVE GVESLKNEIL KALPTE

Show sample condition

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2FS1, Strand ID: A Detail

Release date

2006-09-06

Citation

Structure, dynamics, and stability variation in bacterial albumin binding modules: implications for species specificity
He, Y., Rozak, D., Sari, N., Chen, Y., Bryan, P., Orban, J.
Biochemistry (2006), 45, 10102-10109, PubMed 16906768 , DOI 10.1021/bi060409m , Abstract

Related entities 1. PSD-1, : 1 : 29 entities Detail

Experiments performed 9 experiments Detail

1 1H15N_HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

2 HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

3 CBCACONH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

4 HBHA(CBCACO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

5 HCCONH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

6 HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

7 CCONH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

8 CBHD

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

9 CBHE

Instrument

Model Bruker DRX (600 MHz)

Sample #1

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
1	PSD-1	[U-15N]	0.2 ~ 0.4 mM
2	sodium phosphate		50 mM
3	EDTA		0.1 mM
4	D2O		10 %
5	H2O		90 %

Sample #2

pH 7.0 (±0.1), Details 0.2mM~0.4mM PSD-1 protein + pH 7 50 mM sodium phosphate + 0.1 mM EDTA + 10% D2O.

#	Name	Isotope labeling	Concentration
6	PSD-1	[U-13C; U-15N]	0.2 ~ 0.4 mM
7	sodium phosphate		50 mM
8	EDTA		0.1 mM
9	D2O		10 %
10	H2O		90 %

nullKeywords NMR solution structure, PSD-1

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Found 1 Document (1.571 seconds)

BMRB: 6945

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Sample #2

Related entities 1. PSD-1, : 1 : 29 entities Detail

Experiments performed 9 experiments Detail

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