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BMRB: 6964

2FVT

NMR Structure of the Rpa2829 protein from Rhodopseudomonas palustris: Northeast Structural Genomics Consortium Target RpR43

Authors

Cort, J.R., Kennedy, M.A.

Assembly

Rpa2829

Entity

1. Rpa2829 (polymer, Thiol state: not present), 135 monomers, 15108.06 Da Detail

MAQRSEIPHF PRTAAIDAYG KGGFYFAGMS HQGSLLFLPD AVWGWDVTKP EQIDRYSLQR VFDNANAIDT LIVGTGADVW IAPRQLREAL RGVNVVLDTM QTGPAIRTYN IMIGERRRVA AALIAVPLEH HHHHH

Formula weight

15108.06 Da

Source organism

Rhodopseudomonas palustris

Exptl. method

NMR

Refine. method

The initial structure was determined using automated structure determination (AutoStructure) and refined manually. A final refinement used simultated annealing in explicit solvent.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 85.1 %, Completeness (bb): 91.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.1 % (1323 of 1555)	81.7 % (654 of 800)	87.6 % (538 of 614)	92.9 % (131 of 141)
Backbone	91.5 % (728 of 796)	91.6 % (251 of 274)	91.1 % (359 of 394)	92.2 % (118 of 128)
Sidechain	80.0 % (706 of 883)	76.4 % (402 of 526)	84.9 % (292 of 344)	92.3 % (12 of 13)
Aromatic	54.7 % (82 of 150)	56.0 % (42 of 75)	51.4 % (37 of 72)	100.0 % (3 of 3)
Methyl	96.4 % (160 of 166)	96.4 % (80 of 83)	96.4 % (80 of 83)

1. Rpa2829

MAQRSEIPHF PRTAAIDAYG KGGFYFAGMS HQGSLLFLPD AVWGWDVTKP EQIDRYSLQR VFDNANAIDT LIVGTGADVW IAPRQLREAL RGVNVVLDTM QTGPAIRTYN IMIGERRRVA AALIAVPLEH HHHHH

Show sample condition

Sample

Temperature 293 (±1) K, pH 5.0 (±.1)

#	Name	Isotope labeling	Type	Concentration
1	Rpa2829	[U-13C; U-15N]	protein	1.2 (±0.1) mM
2	ammonium acetate		buffer	20 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	CaCl2		salt	5 (±0.25) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.74 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2FVT, Strand ID: A Detail

Release date

2006-03-28

Citation

NMR structure of the Rpa2829 protein from Rhodopseudomonas palustris
Cort, J.R., Kennedy, M.A.

Related entities 1. Rpa2829, : 1 : 18 entities Detail

Experiments performed 13 experiments Detail

1 1H15N_HSQC