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Found 1 Document (1.917 seconds)

BMRB: 6970

1I4L

1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1

Authors

Bouguet-Bonnet, S., Buck, M.

Assembly

Rac1.GMPPNP GTPase

Entity

1. Rac1 (polymer, Thiol state: all free), 183 monomers, 20294.28 Da Detail

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLSPP PVK

2. GTP (non-polymer), 523.180 Da
3. MG (non-polymer), 24.305 Da

Total weight

20841.764 Da

Max. entity weight

20294.28 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.7 %, Completeness: 42.3 %, Completeness (bb): 69.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.3 % (905 of 2138)	16.1 % (178 of 1107)	68.8 % (584 of 849)	78.6 % (143 of 182)
Backbone	69.3 % (742 of 1070)	41.0 % (149 of 363)	83.6 % (450 of 538)	84.6 % (143 of 169)
Sidechain	25.0 % (310 of 1240)	3.9 % (29 of 744)	58.2 % (281 of 483)	0.0 % (0 of 13)
Aromatic	0.0 % (0 of 156)	0.0 % (0 of 78)	0.0 % (0 of 76)	0.0 % (0 of 2)
Methyl	37.9 % (88 of 232)	9.5 % (11 of 116)	66.4 % (77 of 116)

1. Rac1

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.8 (±0.2), Details The sample is uniformly labeled with 15N and 13C

#	Name	Isotope labeling	Type	Concentration
1	Rac1	[U-15N; U-13C]		0.6 (±0.05) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 6 Detail

Release date

2006-08-06

Citation

1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1
Bouguet-Bonnet, S., Buck, M.
J. Biomol. NMR (2006), 36 Suppl 1, 51-51, PubMed 16858624 , DOI 10.1007/s10858-006-9029-6 , Abstract

Related entities 1. Rac1, : 1 : 61 : 239 entities Detail

Interaction partners 1. Rac1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 7 experiments Detail

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6970_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6970_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	858		89.6 (chain: 1, length: 183)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 183, Sequence coverage: 89.6%
- Total number of assignments
  - ¹³C chemical shifts: 576
  - ¹H chemical shifts: 141
  - ¹⁵N chemical shifts: 141
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	849	576	67.8
¹H chemical shifts	1107	141	12.7
¹⁵N chemical shifts	189	141	74.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	366	300	82.0
¹H chemical shifts	363	141	38.8
¹⁵N chemical shifts	169	141	83.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	483	276	57.1
¹H chemical shifts	744	0	0.0
¹⁵N chemical shifts	20	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	119	74	62.2
¹H chemical shifts	119	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	76	0	0.0
¹H chemical shifts	78	0	0.0
¹⁵N chemical shifts	2	0	0.0

Keywords GTPase, NMR assignment

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Found 1 Document (1.917 seconds)

BMRB: 6970

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Rac1, : 1 : 61 : 239 entities Detail

Interaction partners 1. Rac1, : 5 interactors Detail

Experiments performed 7 experiments Detail

Instrument

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Chemical shift validation 5 contents Detail