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BMRB: 6980

2G2B

Chemical Shift Assignment of human allograft inflammatory factor I

Authors

Song, J., Tyler, R.C., Loushin Newman, C., Vinarov, D., Markley, J.L.

Assembly

BC009474

Entity

1. Allograft inflammatory factor 1 (polymer, Thiol state: not present), 150 monomers, 16968.36 Da Detail

GPLESQTRDL QGGKAFGLLK AQQEERLDEI NKQFLDDPKY SSDEDLPSKL EGFKEKYMEF DLNGNGDIDI MSLKRMLEKL GVPKTHLELK KLIGEVSSGS GETFSYPDFL RMMLGKRSAI LKMILMYEEK AREKEKPTGP PAKKAISELP

2. CA (non-polymer), 40.078 Da

Total weight

17008.438 Da

Max. entity weight

16968.36 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics,simulated annealing,distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 82.2 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.2 % (1487 of 1810)	77.1 % (743 of 964)	86.1 % (600 of 697)	96.6 % (144 of 149)
Backbone	96.3 % (849 of 882)	95.1 % (289 of 304)	96.6 % (422 of 437)	97.9 % (138 of 141)
Sidechain	72.7 % (774 of 1065)	68.9 % (455 of 660)	78.8 % (313 of 397)	75.0 % (6 of 8)
Aromatic	32.3 % (31 of 96)	64.6 % (31 of 48)	0.0 % (0 of 48)
Methyl	91.9 % (125 of 136)	92.6 % (63 of 68)	91.2 % (62 of 68)

1. Allograft inflammatory factor 1

GPLESQTRDL QGGKAFGLLK AQQEERLDEI NKQFLDDPKY SSDEDLPSKL EGFKEKYMEF DLNGNGDIDI MSLKRMLEKL GVPKTHLELK KLIGEVSSGS GETFSYPDFL RMMLGKRSAI LKMILMYEEK AREKEKPTGP PAKKAISELP

Show sample condition

Sample

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Type	Concentration
1	Allograft inflammatory factor 1	protein	0.5 (±0.1) mM
2	MOPS	buffer	10 (±1.0) mM
3	NaCl	salt	100 (±5.0) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.38 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2G2B, Strand ID: A Detail

Release date

2006-03-28

Citation

Solution Structure of Human Allograft Inflammatory Factor I
Song, J., Tyler, R.C., Loushin Newman, C., Vinarov, D., Markley, J.L.

Related entities 1. Allograft inflammatory factor 1, : 1 : 3 : 19 entities Detail

Interaction partners 1. Allograft inflammatory factor 1, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 1H,15N-HSQC