BMRB: 6988

1H, 13C, and 15N Chemical Shift Assignments for HGB1-UBA

Authors

Chang, Y., Song, A., Gao, Y., Shi, Y., Lin, X., Cao, X., Lin, D., Hu, H.

Assembly

HGB1-UBA monomer

Entity

1. HGB1-UBA monomer (polymer, Thiol state: not present), 108 monomers, 11893.91 Da Detail

MHHHHHHQYK LALNGKTLKG ETTTEAVDAA TAEKVFKQYA NDNGVDGEWT YDDATKTFTV TEGSQWQPQL QQLRDMGIQD DELSLRALQA TGGDIQAALE LIFAGGAP

Formula weight

11893.91 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 84.1 %, Completeness (bb): 94.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.1 % (1018 of 1210)	83.4 % (518 of 621)	84.4 % (395 of 468)	86.8 % (105 of 121)
Backbone	94.3 % (607 of 644)	93.8 % (210 of 224)	94.3 % (296 of 314)	95.3 % (101 of 106)
Sidechain	75.9 % (504 of 664)	77.6 % (308 of 397)	76.2 % (192 of 252)	26.7 % (4 of 15)
Aromatic	8.8 % (9 of 102)	15.7 % (8 of 51)	0.0 % (0 of 49)	50.0 % (1 of 2)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. HGB1-UBA

MHHHHHHQYK LALNGKTLKG ETTTEAVDAA TAEKVFKQYA NDNGVDGEWT YDDATKTFTV TEGSQWQPQL QQLRDMGIQD DELSLRALQA TGGDIQAALE LIFAGGAP

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	HGB1-UBA	[U-13C; U-15N]	protein	1 mM
2	PBS		buffer	200 mM
3	NaCl		salt	100 mM

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2DEN, Strand ID: A Detail

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Release date

2006-06-27

Citation

Solution structure of the ubiquitin-associated domain of human BMSC-UbP and its complex with ubiquitin
Chang, Y., Song, A., Gao, Y., Shi, Y., Lin, X., Cao, X., Lin, D., Hu, H.
Protein Sci. (2006), 15, 1248-1259, PubMed 16731964 , DOI 10.1110/ps.051995006 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. HGB1-UBA monomer, : 1 : 1 entities Detail

Experiments performed 4 experiments Detail