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BMRB: 6999

2N5G

KorA

Authors

Rajasekar, K.V., Bingle, L.E.H., Thomas, C.M., Hyde, E.I.

Assembly

KorA dimer

Entity

1. KorA dimer (polymer, Thiol state: not present), 101 monomers, 11305.76 × 2 Da Detail

MKKRLTESQF QEAIQGLEVG QQTIEIARGV LVDGKPQATF ATSLGLTRGA VSQAVHRVWA AFEDKNLPEG YARVTAVLPE HQAYIVRKWE ADAKKKQETK R

Total weight

22611.52 Da

Max. entity weight

11305.76 Da

Source organism

Klebsiella aerogenes

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 86.1 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.1 % (1024 of 1190)	80.1 % (498 of 622)	91.9 % (421 of 458)	95.5 % (105 of 110)
Backbone	97.2 % (583 of 600)	96.6 % (199 of 206)	97.6 % (289 of 296)	96.9 % (95 of 98)
Sidechain	78.1 % (534 of 684)	71.9 % (299 of 416)	87.9 % (225 of 256)	83.3 % (10 of 12)
Aromatic	79.5 % (62 of 78)	84.6 % (33 of 39)	73.0 % (27 of 37)	100.0 % (2 of 2)
Methyl	83.3 % (100 of 120)	86.7 % (52 of 60)	80.0 % (48 of 60)

1. KorA

MKKRLTESQF QEAIQGLEVG QQTIEIARGV LVDGKPQATF ATSLGLTRGA VSQAVHRVWA AFEDKNLPEG YARVTAVLPE HQAYIVRKWE ADAKKKQETK R

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.2 (±0), Details 25 mM Sodium Phosphate buffer at pH 6.2 0.1 mM EDTA

#	Name	Isotope labeling	Concentration
1	KorA	[U-13C; U-15N]	1 mM
2	Phosphate Buffer		25 mM
3	EDTA		0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.77 ppm	external	indirect	0.2514
¹H	Water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	Water	protons	4.77 ppm	external	indirect	0.101329

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.77 ppm	external	indirect	0.2514
¹H	Water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	Water	protons	4.77 ppm	external	indirect	0.101329

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.77 ppm	external	indirect	0.2514
¹H	Water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	Water	protons	4.77 ppm	external	indirect	0.101329

Referencing offset: 0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.77 ppm	external	indirect	0.2514
¹H	Water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	Water	protons	4.77 ppm	external	indirect	0.101329

Referencing offset: -0.29 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N5G, Strand ID: A, B Detail

Release date

2006-09-21

Citation

1H, 13C and 15N assignments of the KorA global transcriptional repressor protein from the low copy number IncP-1 plasmid, RK2
Rajasekar, K.V., Bingle, L.E. H., Thomas, C.M., Hyde, E.I.
J. Biomol. NMR (2006), 36 Suppl 1, 71-71, PubMed 16944275 , DOI 10.1007/s10858-006-9060-7 , Abstract

Related entities 1. KorA dimer, : 1 : 3 : 3 : 1 entities Detail

Interaction partners 1. KorA dimer, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 HNCA