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NMR backbone assignment of the human HSP90 N-terminal domain
Authors
Schwalbe, H., Jacobs, D.M., Elshorst, B., Saxena, K., Fiebig, K.M., Vogtherr, M., Langer, T.
Assembly
hsp90
Entity
1. hsp90 (polymer, Thiol state: not present), 209 monomers, 23316.20 Da Detail

GHVETFAFQA EIAQLMSLII NTFYSNKEIF LRELISNSSD ALDKIRYETL TDPSKLDSGK ELHINLIPNK QDRTLTIVDT GIGMTKADLI NNLGTIAKSG TKAFMEALQA GADISMIGQF GVGFYSAYLV AEKVTVITKH NDDEQYAWES SAGGSFTVRT DTGEPMGRGT KVILHLKEDQ TEYLEERRIK EIVKKHSQFI GYPITLFVE


Formula weight
23316.2 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.3 %, Completeness: 44.3 %, Completeness (bb): 76.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All44.3 % (1083 of 2445)24.2 % (304 of 1258)62.8 % (602 of 958)77.3 % (177 of 229)
Backbone76.0 % (935 of 1230)47.3 % (196 of 414)92.1 % (563 of 611)85.9 % (176 of 205)
Sidechain23.2 % (327 of 1408)12.8 % (108 of 844)40.4 % (218 of 540) 4.2 % (1 of 24)
Aromatic 1.1 % (2 of 188) 2.1 % (2 of 94) 0.0 % (0 of 93) 0.0 % (0 of 1)
Methyl18.0 % (46 of 256)13.3 % (17 of 128)22.7 % (29 of 128)

1. hsp90

GHVETFAFQA EIAQLMSLII NTFYSNKEIF LRELISNSSD ALDKIRYETL TDPSKLDSGK ELHINLIPNK QDRTLTIVDT GIGMTKADLI NNLGTIAKSG TKAFMEALQA GADISMIGQF GVGFYSAYLV AEKVTVITKH NDDEQYAWES SAGGSFTVRT DTGEPMGRGT KVILHLKEDQ TEYLEERRIK EIVKKHSQFI GYPITLFVE

Sample

Temperature 298 (±0.1) K, pH 7.5 (±0.1)


#NameIsotope labelingTypeConcentration
1hsp90[U-2H; U-13C; U-15N]1.5 (±0.2) mM
2sodium phosphate20 (±1.0) mM

LACS Plot; CA
Referencing offset: 2.98 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 2.98 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.31 ppm, Outliers: 1 Detail
Release date
2006-08-06
Citation
NMR backbone assignment of the N-terminal domain of human HSP90
Jacobs, D.M., Langer, T., Elshorst, B., Saxena, K., Fiebig, K.M., Vogtherr, M., Schwalbe, H.
J. Biomol. NMR (2006), 36 Suppl 1, 52-52, PubMed 16821127 , DOI 10.1007/s10858-006-9030-0 ,
Related entities 1. hsp90, : 1 : 8 : 13 : 177 entities Detail
Interaction partners 1. hsp90, : 1 interactors Detail
Experiments performed 6 experiments Detail