BMRB: 7009

First Ca2+ binding domain of the Na,Ca-exchanger (NCX1)

Authors

Hilge, M., Aelen, J., Vuister, G.W.

Assembly

Sodium/calcium exchanger 1

Entity

1. Sodium/calcium exchanger 1 (polymer, Thiol state: all free), 147 monomers, 16193.52 Da Detail

MSHHHHHHVS KIFFEQGTYQ CLENCGTVAL TIIRRGGDLT NTVFVDFRTE DGTANAGSDY EFTEGTVVFK PGETQKEIRV GIIDDDIFEE DENFLVHLSN VKVSSEASED GILEANHVSA LACLGSPSTA TVTIFDDDHA GIFTFEE

2. CA (non-polymer), 40.078 Da

Total weight

16233.598 Da

Max. entity weight

16193.52 Da

Source organism

Canis lupus familiaris

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 79.3 %, Completeness (bb): 77.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.3 % (1286 of 1621)	86.4 % (709 of 821)	68.0 % (439 of 646)	89.6 % (138 of 154)
Backbone	77.6 % (681 of 878)	89.5 % (272 of 304)	64.6 % (277 of 429)	91.0 % (132 of 145)
Sidechain	82.8 % (727 of 878)	84.5 % (437 of 517)	80.7 % (284 of 352)	66.7 % (6 of 9)
Aromatic	51.2 % (83 of 162)	63.0 % (51 of 81)	39.5 % (32 of 81)
Methyl	97.0 % (161 of 166)	95.2 % (79 of 83)	98.8 % (82 of 83)

1. Sodium/calcium exchanger 1

MSHHHHHHVS KIFFEQGTYQ CLENCGTVAL TIIRRGGDLT NTVFVDFRTE DGTANAGSDY EFTEGTVVFK PGETQKEIRV GIIDDDIFEE DENFLVHLSN VKVSSEASED GILEANHVSA LACLGSPSTA TVTIFDDDHA GIFTFEE

Show sample condition

Sample

Pressure 1 atm, Temperature 306 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Sodium/calcium exchanger 1	[U-13C; U-15N]		0.5 ~ 1.0 mM
2	HEPES			20 mM
3	beta-Mercaptoethanol buffer			20 mM
4	H2O			95 %
5	D2O			5 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2FWS, Strand ID: A Detail

---

Release date

2006-04-10

Citation

Ca2+ regulation in the Na+/Ca2+ exchanger involves two markedly different Ca2+ sensors
Hilge, M., Aelen, J., Vuister, G.W.
Mol. Cell (2006), 22, 15-25, PubMed 16600866 , DOI 10.1016/j.molcel.2006.03.008 , Abstract

Related entities 1. Sodium/calcium exchanger 1, : 1 : 1 : 1 : 104 entities Detail

Interaction partners 1. Sodium/calcium exchanger 1, : 1 interactors Detail

Experiments performed 3 experiments Detail