BMRB: 7012

Solution structure of 55-72 segment of staphylococcal nuclease

Authors

Wang, M., Shan, L., Wang, J.F.

Assembly

18-mer peptide from Thermonuclease (E.C.3.1.31.1)

Entity

1. 18-mer peptide from Thermonuclease (E.C.3.1.31.1) (polymer, Thiol state: not present), 18 monomers, 1949.271 Da Detail

GPEASAFTKK MVENAKKI

Formula weight

1949.271 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 77.9 %, Completeness (bb): 78.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	77.9 % (152 of 195)	84.1 % (95 of 113)	69.5 % (57 of 82)
Backbone	78.7 % (70 of 89)	97.2 % (35 of 36)	66.0 % (35 of 53)
Sidechain	80.5 % (99 of 123)	77.9 % (60 of 77)	84.8 % (39 of 46)
Aromatic	30.0 % (3 of 10)	60.0 % (3 of 5)	0.0 % (0 of 5)
Methyl	87.5 % (14 of 16)	87.5 % (7 of 8)	87.5 % (7 of 8)

1. 18-mer peptide from Thermonuclease (E.C.3.1.31.1)

GPEASAFTKK MVENAKKI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	18-mer peptide from Thermonuclease (E.C.3.1.31.1)			2.0 mM
2	DSS			0.02 mM
3	NaN3			0.01 mM
4	trifluoroethanol-d4			40 %
5	H2O			60 %

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Protein Blocks Logo

Calculated from 15 models in PDB: 2FXY, Strand ID: A Detail

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Release date

2006-09-06

Citation

Two peptide fragments G55-I72 and K97-A109 from staphylococcal nuclease exhibit different behaviors in conformational preferences for helix formation
Wang, M., Shan, L., Wang, J.F.
Biopolymers (2006), 83, 268-279, PubMed 16767771 , DOI 10.1002/bip.20558 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. 18-mer peptide from Thermonuclease (E.C.3.1.31.1), : 1 : 104 : 1 entities Detail

Experiments performed 5 experiments Detail