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Backbone 1H, 13C, and 15N Chemical Shift Assignments for calponin homology domain of human MICAL_1
Authors
Dai, H., Sun, H., Zhang, J., Xiong, S., Xu, J., Wu, J., Shi, Y.
Assembly
MICAL 1 CH monomer
Entity
1. MICAL 1 CH monomer (polymer, Thiol state: all free), 118 monomers, 12566.07 Da Detail

MGHHHHHHMG SAGTQEELLR WCQEQTAGYP GVHVSDLSSS WADGLALCAL VYRLQPGLLE PSELQGLGAL EATAWALKVA ENELGITPVV SAQAVVAGSD PLGLIAYLSH FHSAFKSM


Formula weight
12566.07 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.1 %, Completeness: 86.9 %, Completeness (bb): 91.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.9 % (1124 of 1293)89.6 % (588 of 656)81.9 % (421 of 514)93.5 % (115 of 123)
Backbone91.7 % (640 of 698)92.6 % (225 of 243)90.6 % (310 of 342)92.9 % (105 of 113)
Sidechain83.0 % (582 of 701)87.9 % (363 of 413)75.2 % (209 of 278)100.0 % (10 of 10)
Aromatic31.9 % (37 of 116)58.6 % (34 of 58) 0.0 % (0 of 55)100.0 % (3 of 3)
Methyl97.9 % (143 of 146)97.3 % (71 of 73)98.6 % (72 of 73)

1. MICAL 1 CH

MGHHHHHHMG SAGTQEELLR WCQEQTAGYP GVHVSDLSSS WADGLALCAL VYRLQPGLLE PSELQGLGAL EATAWALKVA ENELGITPVV SAQAVVAGSD PLGLIAYLSH FHSAFKSM

Sample #1

Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.01), Details 15N-labeled MICAL_1 CH


#NameIsotope labelingTypeConcentration
1MICAL_1 CH[U-15N]protein1.5 (±0.1) mM
2phosphate buffer50 (±1.0) mM
3NaCl50 (±1.0) mM
4D2O10 (±1.0) %
Sample #2

Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.01), Details 13C, 15N- labeled MICAL-1 CH


#NameIsotope labelingTypeConcentration
5MICAL_1 CH[U-13C; U-15N]protein1.5 (±0.1) mM
6phosphate buffer50 (±1.0) mM
7phosphate buffer50 (±1.0) mM
8D2O10 (±1.0) %
Sample #3

Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.01), Details 13C, 15N- labeled MICAL-1 CH


#NameIsotope labelingTypeConcentration
9MICAL_1 CH[U-13C; U-15N]protein1.5 (±0.1) mM
10phosphate buffer50 (±0.1) mM
11NaCl50 (±0.1) mM
12D2O99.96 (±1.0) %

LACS Plot; CA
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.04 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.04 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2DK9, Strand ID: A Detail


Release date
2006-06-26
Citation
Solution structure of calponin homology domain of Human MICAL-1
Sun, H., Dai, H., Zhang, J., Jin, X., Xiong, S., Xu, J., Wu, J., Shi, Y.
J. Biomol. NMR (2006), 36, 295-300, PubMed 17043746 , DOI 10.1007/s10858-006-9062-5 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: Q8TDZ2 released on 2002-11-08
    Title MICA1_HUMAN Entity Protein-methionine sulfoxide oxidase MICAL1
Related entities 1. MICAL 1 CH monomer, : 1 : 2 : 243 entities Detail
Interaction partners 1. MICAL 1 CH monomer, : 8 interactors Detail
Experiments performed 12 experiments Detail
nullKeywords calponin homology domain, MICAL, NMR structure