Backbone 1H, 13C, and 15N Chemical Shift Assignments for calponin homology domain of human MICAL_1
MGHHHHHHMG SAGTQEELLR WCQEQTAGYP GVHVSDLSSS WADGLALCAL VYRLQPGLLE PSELQGLGAL EATAWALKVA ENELGITPVV SAQAVVAGSD PLGLIAYLSH FHSAFKSM
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.9 % (1124 of 1293) | 89.6 % (588 of 656) | 81.9 % (421 of 514) | 93.5 % (115 of 123) |
Backbone | 91.7 % (640 of 698) | 92.6 % (225 of 243) | 90.6 % (310 of 342) | 92.9 % (105 of 113) |
Sidechain | 83.0 % (582 of 701) | 87.9 % (363 of 413) | 75.2 % (209 of 278) | 100.0 % (10 of 10) |
Aromatic | 31.9 % (37 of 116) | 58.6 % (34 of 58) | 0.0 % (0 of 55) | 100.0 % (3 of 3) |
Methyl | 97.9 % (143 of 146) | 97.3 % (71 of 73) | 98.6 % (72 of 73) |
1. MICAL 1 CH
MGHHHHHHMG SAGTQEELLR WCQEQTAGYP GVHVSDLSSS WADGLALCAL VYRLQPGLLE PSELQGLGAL EATAWALKVA ENELGITPVV SAQAVVAGSD PLGLIAYLSH FHSAFKSMPressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.01), Details 15N-labeled MICAL_1 CH
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MICAL_1 CH | [U-15N] | protein | 1.5 (±0.1) mM |
2 | phosphate buffer | 50 (±1.0) mM | ||
3 | NaCl | 50 (±1.0) mM | ||
4 | D2O | 10 (±1.0) % |
Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.01), Details 13C, 15N- labeled MICAL-1 CH
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | MICAL_1 CH | [U-13C; U-15N] | protein | 1.5 (±0.1) mM |
6 | phosphate buffer | 50 (±1.0) mM | ||
7 | phosphate buffer | 50 (±1.0) mM | ||
8 | D2O | 10 (±1.0) % |
Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.01), Details 13C, 15N- labeled MICAL-1 CH
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | MICAL_1 CH | [U-13C; U-15N] | protein | 1.5 (±0.1) mM |
10 | phosphate buffer | 50 (±0.1) mM | ||
11 | NaCl | 50 (±0.1) mM | ||
12 | D2O | 99.96 (±1.0) % |