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BMRB: 7080

2G9J

Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ("overlap region")of tropomyosin

Authors

Greenfield, N.J., Huang, Y.J., Swapna, G., Bhattacharya, A., Rapp, B., Singh, A., Montelione, G.T., Hitchcock-DeGregori, S.E.

Assembly

NTD-CTD complex

Entity

1. Tropomyosin N-terminal domain (polymer, Thiol state: not present), 33 monomers, 3900.635 × 2 Da Detail

GMDAIKKKMQ MLKLDNYHLE NEVARLKKLV GER

2. Tropomyosin C-terminal domain (polymer, Thiol state: all disulfide bound), 37 monomers, 4171.656 × 2 Da Detail

GCGKSIDDLE DELYAQKLKY KAISEELDHA LKDMTSI

Total weight

16144.582 Da

Max. entity weight

4171.656 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	2:CYS2:SG	2:CYS2:SG

Source organism

Rattus rattus

Exptl. method

NMR

Refine. method

Initail structure was calculated with Torsion Angle Dynamics and refined with simulated annealing and included a term for explicit solvent in the refinement protocol.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.0 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.0 % (808 of 842)	97.5 % (434 of 445)	93.5 % (302 of 323)	97.3 % (72 of 74)
Backbone	97.4 % (409 of 420)	97.2 % (140 of 144)	97.6 % (201 of 206)	97.1 % (68 of 70)
Sidechain	95.3 % (465 of 488)	97.7 % (294 of 301)	91.3 % (167 of 183)	100.0 % (4 of 4)
Aromatic	46.9 % (15 of 32)	93.8 % (15 of 16)	0.0 % (0 of 16)
Methyl	97.4 % (74 of 76)	97.4 % (37 of 38)	97.4 % (37 of 38)

1. Tropomyosin N-terminal domain

GMDAIKKKMQ MLKLDNYHLE NEVARLKKLV GER

2. Tropomyosin C-terminal domain

GCGKSIDDLE DELYAQKLKY KAISEELDHA LKDMTSI

Show sample condition

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Protein Blocks Logo

Calculated from 10 models in PDB: 2G9J, Strand ID: A, B, C, D Detail

Release date

2006-10-30

Citation 1

Solution NMR structure of the junction between tropomyosin molecules: implications for actin binding and regulation
Greenfield, N.J., Huang, Y.J., Swapna, G., Bhattacharya, A., Rapp, B., Singh, A., Montelione, G.T., Hitchcock-DeGregori, S.E.
J. Mol. Biol. (2006), 364, 80-96, PubMed 16999976 , DOI 10.1016/j.jmb.2006.08.033 , Abstract

Show more 6 citaions

Citation 2

A topology-constrained distance network algorithm for protein structure determination from NOESY data
Huang, Y.J., Tejero, R., Powers, R., Montelione, G.T.
Proteins (2006), 62, 587-603, PubMed 16374783 , DOI 10.1002/prot.20820 , Abstract

Citation 3

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, Mumenthaler, Wuthrich
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Citation 4

Automated NMR structure calculation with CYANA
Guntert
Methods. Mol. Biol. (2004), 278, 353-378, PubMed 15318003 , DOI 10.1385/1-59259-809-9:353 , Abstract

Citation 5

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 6

Crystallography & NMR system: A new software suite for macromolecular structure determination
Brunger, A.T., Adams, P.D., Clore, DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T., Warren, G.L.
Acta Crystallogr. D. Biol. Crystallogr. (1998), 54, 905-921, PubMed 9757107 , Abstract

Citation 7

SPARKY 3
Goddard, T.D., Kneller, D.G.

Hide non-primary 6 citaions

Related entities 1. Tropomyosin N-terminal domain, : 7 entities Detail

Related entities 2. Tropomyosin C-terminal domain, : 1 : 1 : 15 : 68 entities Detail

Interaction partners 2. Tropomyosin C-terminal domain, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 19 experiments Detail

1 1H15N_HSQC

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

2 1H13C_HSQC

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

3 HNCA

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

4 HNcoCA

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

5 HCCH_COSY

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

6 CCH-COSY

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

7 HNCO

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

8 HAcaNH

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

9 HAcacoNH

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

10 CBCAcoNH

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

11 CBCANH

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

12 15N Edited NOESY

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

13 13C Edited NOESY

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

14 13C X-filtered NOESY

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

15 T1 Relaxation

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

16 T2 Relaxation

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

17 15N1H HNOE

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

18 15N Edited TOCSY

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

19 HNcoHA

Instrument

#1 Model Varian ANOVA (600 MHz)

#2 Model Varian Anova (500 MHz)

#3 Model Bruker AVANCE (600 MHz) Details Has cryoprobe

Sample #1

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex both chains labeled with 15N, in 100 mM NaCl, 10 mM NaPO4, 7.5% D2O pH*=6.5

#	Name	Isotope labeling	Type	Concentration
1	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
2	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
3	NaCl		salt	100 (±10.0) mM
4	Sodium Phosphate		buffer	10 (±1.0) mM
5	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #2

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
6	Tropomyosin C-terminal domain	[U-95% 15N U-95% 13C]	protein	1.0 (±0.1) mM
7	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
8	NaCl		salt	100 (±10.0) mM
9	Sodium Phosphate		buffer	10 (±1.0) mM
10	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #3

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
11	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
12	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
13	NaCl		salt	100 (±10.0) mM
14	Sodium Phosphate		buffer	10 (±1.0) mM
15	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #4

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex CTD labeled with 13C/15N NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
16	Tropomyosin C-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
17	Tropomyosin N-terminal domain	[U-95% 15N]	protein	1.0 (±0.1) mM
18	NaCl		salt	100 (±10.0) mM
19	Sodium Phosphate		buffer	10 (±1.0) mM
20	Deuterium Oxide	[U-2H]	solvent	99 (±1.0) %

Sample #5

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex NTD labeled with 13C/15N CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
21	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
22	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	1.0 (±0.1) mM
23	NaCl		salt	100 (±10.0) mM
24	Sodium Phosphate		buffer	10 (±1.0) mM
25	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #6

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of CTD labeled with 13C/15N CTD one chain of CTD unlabeled both chains of NTD unlabeled

#	Name	Isotope labeling	Type	Concentration
26	Tropomyosin C-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
27	Tropomyosin C-terminal domain	unlabeled	protein	0.5 (±0.05) mM
28	Tropomyosin N-terminal domain	unlabeled	protein	1.0 (±0.1) mM
29	NaCl		salt	100 (±10.0) mM
30	Sodium Phosphate		buffer	10 (±1.0) mM
31	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

Sample #7

Pressure 1 (±0.05) atm, Temperature 283 (±0.1) K, Details NTD-CTD complex one chain of NTD labeled with 13C/15N CTD one chain of NTD unlabeled both chains of CTD unlabeled

#	Name	Isotope labeling	Type	Concentration
32	Tropomyosin C-terminal domain	unlabeled	protein	1.0 (±0.1) mM
33	Tropomyosin N-terminal domain	[U-95% 15N; U-95% 13C]	protein	0.5 (±0.05) mM
34	Tropomyosin N-terminal domain	unlabeled	protein	0.5 (±0.05) mM
35	NaCl		salt	100 (±10.0) mM
36	Sodium Phosphate		buffer	10 (±1.0) mM
37	Deuterium Oxide	[U-2H]	solvent	7.5 (±2.5) %

nullKeywords intermolecular junction, N-terminal C-terminal Interface, overlap complex, parallel coiled coil, peptide complex, Polymerization domain, tropomyosin, AutoStructure, DYANA, CYANA, NMRPipe, CNS, Sparky

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Found 1 Document (2.832 seconds)

BMRB: 7080

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Sample #7

Related entities 1. Tropomyosin N-terminal domain, : 7 entities Detail

Related entities 2. Tropomyosin C-terminal domain, : 1 : 1 : 15 : 68 entities Detail

Interaction partners 2. Tropomyosin C-terminal domain, : 2 interactors Detail

Experiments performed 19 experiments Detail

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