BMRB: 7083

Chemical shifts of SBD from Rhizopu oryzae glucoamylase

Authors

Liu, Y., Lyu, P.

Assembly

SBD

Entity

1. SBD (polymer, Thiol state: not present), 106 monomers, 11654.51 Da Detail

ASIPSSASVQ LDSYNYDGST FSGKIYVKNI AYSKKVTVVY ADGSDNWNNN GNIIAASFSG PISGSNYEYW TFSASVKGIK EFYIKYEVSG KTYYDNNNSA NYQVST

Formula weight

11654.51 Da

Source organism

Rhizopus oryzae

Exptl. method

NMR

Refine. method

torsion angle dynamics, distance geometry, simulated annealing, water refine

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.8 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.8 % (973 of 1204)	87.5 % (538 of 615)	67.4 % (316 of 469)	99.2 % (119 of 120)
Backbone	98.9 % (625 of 632)	98.2 % (214 of 218)	99.4 % (308 of 310)	99.0 % (103 of 104)
Sidechain	66.4 % (445 of 670)	81.6 % (324 of 397)	40.9 % (105 of 257)	100.0 % (16 of 16)
Aromatic	30.0 % (48 of 160)	57.5 % (46 of 80)	0.0 % (0 of 78)	100.0 % (2 of 2)
Methyl	64.9 % (61 of 94)	100.0 % (47 of 47)	29.8 % (14 of 47)

1. SBD

ASIPSSASVQ LDSYNYDGST FSGKIYVKNI AYSKKVTVVY ADGSDNWNNN GNIIAASFSG PISGSNYEYW TFSASVKGIK EFYIKYEVSG KTYYDNNNSA NYQVST

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SBD			1 mM

Sample #2

Temperature 298 (±0.1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	SBD	[U-98% 15N]		1 mM

Sample #3

Temperature 298 (±0.1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SBD	[U-98% 15N; U-98% 13C]		1 mM

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LACS Plot; CA

Referencing offset: 2.23 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.23 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2DJM, Strand ID: A Detail

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Release date

2007-05-01

Citation

Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase
Liu, Y., Lai, Y., Chou, W., Chang, M.D., Lyu, P.
Biochem. J. (2007), 403, 21-30, PubMed 17117925 , DOI 10.1042/BJ20061312 , Abstract

Related entities 1. SBD, : 1 : 5 : 1 : 42 entities Detail

Experiments performed 5 experiments Detail