BMRB: 7114

1H, 13C, and 15N chemical shift assignment of Escherichia coli Maltose Binding Protein complexed with Beta-Cyclodextrin

Authors

Xu, Y., Lin, Z., Zheng, Y., Yang, D.

Assembly

E. coli maltose binding protein

Entity

1. maltose binding protein (polymer, Thiol state: not present), 370 monomers, 40694.71 Da Detail

KTEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPW AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK

2. BCD (polymer), 1134.984 Da

Total weight

41829.695 Da

Max. entity weight

40694.71 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.6 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.6 % (3998 of 4316)	89.5 % (2002 of 2237)	95.7 % (1620 of 1692)	97.2 % (376 of 387)
Backbone	97.8 % (2129 of 2178)	97.6 % (730 of 748)	97.5 % (1054 of 1081)	98.9 % (345 of 349)
Sidechain	89.0 % (2207 of 2479)	85.4 % (1272 of 1489)	95.0 % (904 of 952)	81.6 % (31 of 38)
Aromatic	84.7 % (320 of 378)	85.2 % (161 of 189)	83.4 % (151 of 181)	100.0 % (8 of 8)
Methyl	98.8 % (413 of 418)	98.6 % (206 of 209)	99.0 % (207 of 209)

1. maltose binding protein

KTEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPW AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK

Show sample condition

Sample

Temperature 303 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	maltose binding protein			1.2 mM
2	beta-cyclodextrin

Hide sample codition

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Calculated from 10 models in PDB: 2H25, Strand ID: A Detail

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Release date

2006-10-31

Citation

A new strategy for structure determination of large proteins in solution without deuteration
Xu, Y., Zheng, Y., Fan, J., Yang, D.
Nat. Methods (2006), 3, 931-937, PubMed 17060917 , DOI 10.1038/nmeth938 , Abstract

Related entities 1. maltose binding protein, : 1 : 3 : 97 : 13 entities Detail

Interaction partners 1. maltose binding protein, : 1 interactors Detail

Experiments performed 4 experiments Detail