1H,13C and 15N resonance assignments of barnase-barstar complex NMR deuterium methyl relaxation data for free barnase and barnase-barstar complex
AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 25.3 % (594 of 2348) | 16.8 % (205 of 1217) | 30.7 % (280 of 912) | 49.8 % (109 of 219) |
Backbone | 38.5 % (456 of 1184) | 29.7 % (121 of 408) | 39.0 % (227 of 582) | 55.7 % (108 of 194) |
Sidechain | 17.4 % (235 of 1348) | 10.4 % (84 of 809) | 29.2 % (150 of 514) | 4.0 % (1 of 25) |
Aromatic | 0.0 % (0 of 224) | 0.0 % (0 of 112) | 0.0 % (0 of 106) | 0.0 % (0 of 6) |
Methyl | 50.5 % (112 of 222) | 50.5 % (56 of 111) | 50.5 % (56 of 111) |
1. barnase
AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR2. barstar
KKAVINGEQI RSISDLHQTL KKELALPEYY GENLDALWDC LTGWVEYPLV LEWRQFEQSK QLTENGAESV LQVFREAKAE GCDITIILSTemperature 303 (±0.2) K, pH 6.7 (±0.5)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | barnase | [U-13C; U-15N; U-40% 2H] | 1.17 mM | |
2 | barstar | 1.17 mM |