BMRB: 7144

Chemical shift changes upon ligand binding

Authors

Kristiansen, A., Varum, K.M., Grasdalen, H.

Assembly

HEWL

Entity

1. HEWL (polymer, Thiol state: unknown), 129 monomers, 14313.99 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT EATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Formula weight

14313.99 Da

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 13.2 %, Completeness: 6.3 %, Completeness (bb): 5.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	6.3 % (47 of 750)	6.3 % (47 of 750)
Backbone	5.2 % (14 of 268)	5.2 % (14 of 268)
Sidechain	6.8 % (33 of 482)	6.8 % (33 of 482)
Aromatic	32.3 % (21 of 65)	32.3 % (21 of 65)
Methyl	6.8 % (4 of 59)	6.8 % (4 of 59)

1. hen egg white lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT EATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Show sample condition

Sample

Temperature 308 (±0) K

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme			3 mM
2	NaCl			0.15 M
3	H2O			90 %
4	D2O			10 %

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Release date

2010-08-15

Citation

The interactions between highly de-N-acetylated chitosans and lysozyme from chicken egg white studied by 1H-NMR spectroscopy
Kristiansen, A., Varum, K.M., Grasdalen, H.
Eur. J. Biochem. (1998), 251, 335-342, PubMed 9492302 , DOI: , Abstract

Related entities 1. HEWL, : 1 : 106 : 141 entities Detail

Interaction partners 1. HEWL, : 7 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail