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Chemical shift changes upon ligand binding
Authors
Kristiansen, A., Varum, K.M., Grasdalen, H.
Assembly
HEWL
Entity
1. HEWL (polymer, Thiol state: unknown), 129 monomers, 14313.99 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT EATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL


Formula weight
14313.99 Da
Source organism
Gallus gallus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 13.2 %, Completeness: 5.2 %, Completeness (bb): 5.5 % Detail

Polymer type: polypeptide(L)

Total1H
All 5.2 % (39 of 750) 5.2 % (39 of 750)
Backbone 5.5 % (14 of 256) 5.5 % (14 of 256)
Sidechain 5.1 % (25 of 494) 5.1 % (25 of 494)
Aromatic26.2 % (17 of 65)26.2 % (17 of 65)
Methyl 5.1 % (3 of 59) 5.1 % (3 of 59)

1. hen egg white lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT EATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Sample

Temperature 308 (±0) K


#NameIsotope labelingTypeConcentration
1hen egg white lysozyme3 mM
2NaCl0.15 M
3H2O90 %
4D2O10 %

Release date
2010-08-15
Citation
The interactions between highly de-N-acetylated chitosans and lysozyme from chicken egg white studied by 1H-NMR spectroscopy
Kristiansen, A., Varum, K.M., Grasdalen, H.
Eur. J. Biochem. (1998), 251, 335-342, PubMed 9492302 , DOI 10.1046/j.1432-1327.1998.2510335.x ,
Related entities 1. HEWL, : 1 : 104 : 98 entities Detail
Interaction partners 1. HEWL, : 8 interactors Detail
Experiments performed 2 experiments Detail