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NMR spectroscopy of T4 Lysozyme peptide fragments
Authors
Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.
Assembly
HELIX H
Entity
1. HELIX H (polymer, Thiol state: not present), 22 monomers, 2653.950 Da Detail

SRWYNQTPNR AKRVITTFRT GT


Formula weight
2653.95 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

Total1H
All93.0 % (132 of 142)93.0 % (132 of 142)
Backbone95.3 % (41 of 43)95.3 % (41 of 43)
Sidechain91.9 % (91 of 99)91.9 % (91 of 99)
Aromatic100.0 % (15 of 15)100.0 % (15 of 15)
Methyl100.0 % (10 of 10)100.0 % (10 of 10)

1. helix H (lys 136-157)

SRWYNQTPNR AKRVITTFRT GT

Sample

Temperature 283 (±0) K


#NameIsotope labelingTypeConcentration
1helix H (lys 136-157)1.0 ~ 2.0 mM
2H2O90 %
3D2O10 %

Chem. Shift Complete2
Sequence coverage: 100.0 %, Completeness: 93.3 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

Total1H
All93.3 % (265 of 284)93.3 % (265 of 284)
Backbone95.3 % (82 of 86)95.3 % (82 of 86)
Sidechain92.4 % (183 of 198)92.4 % (183 of 198)
Aromatic100.0 % (30 of 30)100.0 % (30 of 30)
Methyl100.0 % (20 of 20)100.0 % (20 of 20)

1. helix H (lys 136-157)

SRWYNQTPNR AKRVITTFRT GT

Sample

Temperature 283 (±0) K


#NameIsotope labelingTypeConcentration
4helix H (lys 136-157)1.0 ~ 2.0 mM
5TFE50 %
6H2O40 %
7D2O10 %

Release date
2008-11-11
Citation
Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments
Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.
Biochemistry (2000), 39, 5911-5920, PubMed 10801343 , DOI 10.1021/bi000070i ,
Related entities 1. HELIX H, : 1 : 99 : 1 : 2 entities Detail
Experiments performed 1 experiments Detail