BMRBj - BMREntryMaster

Found 1 Document (1.328 seconds)

BMRB: 7156

1SSW

NMR spectroscopy of T4 Lysozyme peptide fragments

Authors

Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.

Assembly

HELIX H

Entity

1. HELIX H (polymer, Thiol state: not present), 22 monomers, 2653.950 Da Detail

SRWYNQTPNR AKRVITTFRT GT

Formula weight

2653.95 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	93.0 % (132 of 142)	93.0 % (132 of 142)
Backbone	95.5 % (42 of 44)	95.5 % (42 of 44)
Sidechain	91.8 % (90 of 98)	91.8 % (90 of 98)
Aromatic	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (10 of 10)	100.0 % (10 of 10)

1. helix H (lys 136-157)

SRWYNQTPNR AKRVITTFRT GT

Show sample condition

Sample

Temperature 283 (±0) K

#	Name	Concentration
1	helix H (lys 136-157)	1.0 ~ 2.0 mM
2	H2O	90 %
3	D2O	10 %

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 93.7 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	93.7 % (266 of 284)	93.7 % (266 of 284)
Backbone	96.6 % (85 of 88)	96.6 % (85 of 88)
Sidechain	92.3 % (181 of 196)	92.3 % (181 of 196)
Aromatic	100.0 % (30 of 30)	100.0 % (30 of 30)
Methyl	100.0 % (20 of 20)	100.0 % (20 of 20)

1. helix H (lys 136-157)

SRWYNQTPNR AKRVITTFRT GT

Show sample condition

Sample

Temperature 283 (±0) K

#	Name	Concentration
4	helix H (lys 136-157)	1.0 ~ 2.0 mM
5	TFE	50 %
6	H2O	40 %
7	D2O	10 %

Release date

2008-11-11

Citation

Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments
Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.
Biochemistry (2000), 39, 5911-5920, PubMed 10801343 , DOI: , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 7152 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7153 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7155 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7157 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7154 released on 2006-06-06
    Title NMR spectroscopy of T4 Lysozyme peptide fragments

Related entities 1. HELIX H, : 1 : 99 : 1 : 4 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7156_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7156_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	155	100.0 (chain: 1, length: 22)
2	Assigned chemical shifts	chem_shift_list_2	Warning	157	100.0 (chain: 1, length: 22)
1	Chemical shift references	chemical_shift_referencing	OK	1	No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 22, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 155
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 22, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 157
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
2	ARG	HH11	7.2
10	ARG	HH11	7.2
19	ARG	HH11	7.2

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
2	ARG	HH11	7.26
10	ARG	HH11	7.26
13	ARG	HH11	7.3
19	ARG	HH11	7.26

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Found 1 Document (1.328 seconds)

BMRB: 7156

Sample

Sample

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. HELIX H, : 1 : 99 : 1 : 4 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail