BMRBj - BMREntryMaster

Found 1 Document (1.356 seconds)

BMRB: 7160

2A6U

1H chemical shift assignments of metLYZ at pH 3.8 and 25 C

Authors

Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.

Assembly

metLYZ

Entity

1. metLYZ (polymer, Thiol state: unknown), 129 monomers, 14313.01 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Formula weight

14313.01 Da

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 92.3 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	92.3 % (694 of 752)	92.3 % (694 of 752)
Backbone	98.1 % (263 of 268)	98.1 % (263 of 268)
Sidechain	89.0 % (431 of 484)	89.0 % (431 of 484)
Aromatic	100.0 % (65 of 65)	100.0 % (65 of 65)
Methyl	94.9 % (56 of 59)	94.9 % (56 of 59)

1. metLYZ

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Show sample condition

Sample

Temperature 298 (±0) K, pH 3.8 (±0)

#	Name	Concentration
1	metLYZ	1 mM
2	H2O	95 %
3	D2O	5 %

Release date

2008-11-11

Citation

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 7159 released on 2008-11-11
    Title 1H chemical shift assignments of 2SS[6-127, 30-115] at pH 3.8 and 25 C
  BMRB: 6415 released on 2005-04-03
    Title Three-disulfide variant of hen lysozyme: C30A/C115A
  BMRB: 5803 released on 2004-09-13
    Title Three-disulfide variant of hen lysozyme: C64A/C80A
  BMRB: 5804 released on 2004-09-13
    Title Three-disulfide variant of hen lysozyme: C76A/C94A
  BMRB: 5068 released on 2002-02-26
    Title Recombinant hen lysozyme containing the extra N-terminal Met as the standard reference for the study of hen lysozyme variants
  BMRB: 5069 released on 2002-02-26
    Title NMR Structural Study of Two-Disulfide Variant of hen Lysozyme: 2SS[6-127, 30-115]-A Disulfide Intermediate with a Partly Unfolded Structure

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7160_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	802		99.2 (chain: 1, length: 129)
1	Chemical shift references	chemical_shift_referencing	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
15	HIS	HD1	7.54
15	HIS	HE2	8.86
33	LYS	HZ1	7.33
33	LYS	HZ2	7.33
33	LYS	HZ3	7.33

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Found 1 Document (1.356 seconds)

BMRB: 7160

Sample

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. metLYZ, : 1 : 105 : 1 : 141 entities Detail

Interaction partners 1. metLYZ, : 7 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail