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BMRB: 7161

Backbone 1H, 13C and 15N Chemical Shift Assignments of Ezrin C ERMAD in a non-covalent complex with Ezrin N FERM

Authors

Jayaraman, B., Nicholson, L.K.

Assembly

Ezrin FERM C ERMAD Complex

Entity

1. C ERMAD (polymer, Thiol state: not present), 112 monomers, 13140.15 Da Detail

PPVYEPVSYH VQESLQDEGA EPTGYSAELS SEGIRDDRNE EKRITEAEKN ERVQRQLLTL SSELSQARDE NKRTHNDIIH NENMRQGRDK YKTLRQIRQG NTKQRIDEFE AL

2. N FERM (polymer, Thiol state: unknown), 295 monomers, 34797.82 Da Detail

PKPINVRVTT MDAELEFAIQ PNTTGKQLFD QVVKTIGLRE VWYFGLHYVD NKGFPTWLKL DKKVSAQEVR KENPLQFKFR AKFYPEDVAE ELIQDITQKL FFLQVKEGIL SDEIYCPPET AVLLGSYAVQ AKFGDYNKEV HKSGYLSSER LIPQRVMDQH KLTRDQWEDR IQVWHAEHRG MLKDNAMLEY LKIAQDLEMY GINYFEIKNK KGTDLWLGVD ALGLNIYEKD DKLTPKIGFP WSEIRNISFN DKKFVIKPID KKAPDFVFYA PRLRINKRIL QLCMGNHELY MRRRK

Total weight

47937.97 Da

Max. entity weight

34797.82 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 28.7 %, Completeness: 11.5 %, Completeness (bb): 21.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	11.5 % (579 of 5028)	5.9 % (158 of 2657)	16.4 % (317 of 1932)	23.7 % (104 of 439)
Backbone	21.9 % (526 of 2406)	14.2 % (116 of 816)	25.5 % (306 of 1201)	26.7 % (104 of 389)
Sidechain	4.9 % (147 of 3009)	2.3 % (43 of 1841)	9.3 % (104 of 1118)	0.0 % (0 of 50)
Aromatic	0.0 % (0 of 414)	0.0 % (0 of 207)	0.0 % (0 of 201)	0.0 % (0 of 6)
Methyl	3.9 % (16 of 412)	2.9 % (6 of 206)	4.9 % (10 of 206)

1. C ERMAD

PPVYEPVSYH VQESLQDEGA EPTGYSAELS SEGIRDDRNE EKRITEAEKN ERVQRQLLTL SSELSQARDE NKRTHNDIIH NENMRQGRDK YKTLRQIRQG NTKQRIDEFE AL

2. N FERM

Show sample condition

Sample

Temperature 303 (±2) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C ERMAD	[U-99% 13C; U-99% 15N; U-95% 2H]	protein	0.5 mM
2	N FERM	natural abundance	protein	0.5 mM
3	Acetic Acid	natural abundance	buffer	10 mM
4	Sodium Acetate	natural abundance	buffer	90 mM
5	DTT	natural abundance	buffer	5 mM
6	Sodium Azide	natural abundance	buffer	15 mM
7	Protease inhibitors	natural abundance		0.0 ~ 0.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

Release date

2006-10-01

Citation

Backbone resonance assignments of Ezrin C ERMAD in a non-covalent complex with Ezrin N FERM
Jayaraman, B., Nicholson, L.K.
J. Biomol. NMR (2006), 36 Suppl 1, 63-63, PubMed 17131035 , DOI 10.1007/s10858-006-9049-2 , Abstract

Related entities 1. C ERMAD, : 2 : 20 entities Detail

Related entities 2. N FERM, : 1 : 94 entities Detail

Interaction partners 1. C ERMAD, : 51 interactors Detail

More details for 51 interactors identified by 30 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC