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Found 1 Document (4.336 seconds)

BMRB: 7225

2HEP

Solution NMR structure of the UPF0291 protein ynzC from Bacillus subtilis. Northeast Structural Genomics target SR384. (CASP Target)

Authors

Rost, B., Aramini, J.M., Swapna, G.V.T., Ho, C.K., Shetty, K., Cunningham, K., Ma, L., Xiao, R., Liu, J., Baran, M., Acton, T.B., Montelione, G.T.

Assembly

UPF0291 protein ynzC

Entity

1. UPF0291 protein ynzC (polymer, Thiol state: not present), 85 monomers, 9871.153 Da Detail

MISNAKIARI NELAAKAKAG VITEEEKAEQ QKLRQEYLKG FRSSMKNTLK SVKIIDPEGN DVTPEKLKRE QRNNKLHLEH HHHHH

Formula weight

9871.153 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 80.5 %, Completeness (bb): 87.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.5 % (834 of 1036)	75.2 % (415 of 552)	87.7 % (343 of 391)	81.7 % (76 of 93)
Backbone	87.2 % (441 of 506)	85.4 % (146 of 171)	88.5 % (223 of 252)	86.7 % (72 of 83)
Sidechain	76.1 % (466 of 612)	70.6 % (269 of 381)	87.3 % (193 of 221)	40.0 % (4 of 10)
Aromatic	39.1 % (18 of 46)	39.1 % (9 of 23)	39.1 % (9 of 23)
Methyl	92.9 % (78 of 84)	85.7 % (36 of 42)	100.0 % (42 of 42)

1. UPF0291 protein ynzC

MISNAKIARI NELAAKAKAG VITEEEKAEQ QKLRQEYLKG FRSSMKNTLK SVKIIDPEGN DVTPEKLKRE QRNNKLHLEH HHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2HEP, Strand ID: A Detail

Release date

2006-07-13

Citation

Solution NMR structure of the SOS response protein YnzC from Bacillus subtilis
Aramini, J.M., Sharma, S., Huang, Y.J., Swapna, G.V.T., Ho, C.K., Shetty, K., Cunningham, K., Ma, L., Zhao, L., Owens, L.A., Jiang, M., Xiao, R., Liu, J., Baran, M.C., Acton, T.B., Rost, B., Montelione, G.T.
Proteins (2008), 72, 526-530, PubMed 18431750 , DOI 10.1002/prot.22064 , Abstract

Related entities 1. UPF0291 protein ynzC, : 1 : 1 : 43 entities Detail

Experiments performed 13 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

2 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

3 HNHA

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

4 3D GFT-CBCACAcoNHN

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

5 GFT-HNNCACBCA

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

6 GFT-HABCABcoNHN

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

7 3D HCCH-TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

8 CCcoNH TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

9 HCCH-COSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

10 high resolution 2D CH-HQSC (for stereospecific assignment of Val/Leu methyls)

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

11 3D HNCO

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

12 HNcaCO

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

13 2D 15N1H-heteronuclear NOE

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UPF0291 protein ynzC	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	UPF0291 protein ynzC	[U-5% 13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

nullKeywords AUTOSTRUCTURE, NESG, NMR structure, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI-1, SR384

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Found 1 Document (4.336 seconds)

BMRB: 7225

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. UPF0291 protein ynzC, : 1 : 1 : 43 entities Detail

Experiments performed 13 experiments Detail

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