NMR Investigation of Tyr105 Mutants in TEM-1 beta-lactamase Suggests Active-Site Dynamical Coupling
HPETLVKVKD AEDQLGARVG YIELDLNSGK ILESFRPEER FPMMSTFKVL LCGAVLSRVD AGQEQLGRRI HYSQNDLVED SPVTEKHLTD GMTVRELCSA AITMSDNTAA NLLLTTIGGP KELTAFLHNM GDHVTRLDRW EPELNEAIPN DERDTTMPAA MATTLRKLLT GELLTLASRQ QLIDWMEADK VAGPLLRSAL PAGWFIADKS GAGERGSRGI IAALGPDGKP SRIVVIYTTG SQATMDERNR QIAEIGASLI KHW
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS52:SG | 1:CYS98:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.3 % (930 of 2968) | 21.7 % (334 of 1537) | 30.0 % (348 of 1160) | 91.5 % (248 of 271) |
Backbone | 54.2 % (843 of 1554) | 51.4 % (275 of 535) | 41.7 % (320 of 768) | 98.8 % (248 of 251) |
Sidechain | 7.1 % (118 of 1656) | 5.9 % (59 of 1002) | 9.3 % (59 of 634) | 0.0 % (0 of 20) |
Aromatic | 5.5 % (8 of 146) | 5.5 % (4 of 73) | 5.8 % (4 of 69) | 0.0 % (0 of 4) |
Methyl | 7.7 % (25 of 326) | 6.7 % (11 of 163) | 8.6 % (14 of 163) |
1. TEM-1
HPETLVKVKD AEDQLGARVG YIELDLNSGK ILESFRPEER FPMMSTFKVL LCGAVLSRVD AGQEQLGRRI HYSQNDLVED SPVTEKHLTD GMTVRELCSA AITMSDNTAA NLLLTTIGGP KELTAFLHNM GDHVTRLDRW EPELNEAIPN DERDTTMPAA MATTLRKLLT GELLTLASRQ QLIDWMEADK VAGPLLRSAL PAGWFIADKS GAGERGSRGI IAALGPDGKP SRIVVIYTTG SQATMDERNR QIAEIGASLI KHWTemperature 303 (±0.2) K, pH 6.6 (±0.2), Details 0.8 mM, pH 6.6 for all Y105X mutants
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TEM-1 (Y105D) | [U-13C; U-15N] | 0.8 mM | |
2 | imidazole | 4 mM | ||
3 | DSS | 0.1 mM | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |