BMRB: 7257

Solution structure of the RRM domain of SR rich factor 9G8

Authors

Tintaru, A.M., Hautbergue, G.M., Golovanov, A.P., Lian, L.Y., Wilson, S.A.

Assembly

RRM domain of SR rich factor 9G8

Entity

1. RRM domain of SR rich factor 9G8 (polymer, Thiol state: all free), 101 monomers, 11320.84 Da Detail

MKVYVGNLGT GAGKGELERA FSYYGPLRTV WIARNPPGFA FVEFEDPRDA EDAVRGLDGK VICGSRVRVE LSTGMPRRSR FDRPPARRKL LEVLFNGPLE H

Formula weight

11320.84 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 96.8 %, Completeness (bb): 98.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.8 % (1137 of 1175)	96.4 % (596 of 618)	96.7 % (445 of 460)	99.0 % (96 of 97)
Backbone	98.3 % (580 of 590)	98.5 % (203 of 206)	97.9 % (285 of 291)	98.9 % (92 of 93)
Sidechain	95.5 % (644 of 674)	95.4 % (393 of 412)	95.7 % (247 of 258)	100.0 % (4 of 4)
Aromatic	90.0 % (90 of 100)	90.0 % (45 of 50)	89.8 % (44 of 49)	100.0 % (1 of 1)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. RRM domain of SR rich factor 9G8

MKVYVGNLGT GAGKGELERA FSYYGPLRTV WIARNPPGFA FVEFEDPRDA EDAVRGLDGK VICGSRVRVE LSTGMPRRSR FDRPPARRKL LEVLFNGPLE H

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	RRM domain of SR rich factor 9G8	[U-13C; U-15N]		0.6 mM
2	CH3COONa			20 mM
3	L-ARG			25 mM
4	L-Glu			50 mM
5	EDTA			5 mM
6	DTT			10 mM
7	H2O			90 %
8	D2O			10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2HVZ, Strand ID: A Detail

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Release date

2006-12-19

Citation

Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8
Hargous, Y., Hautbergue, G.M., Tintaru, A.M., Skrisovska, L., Golovanov, A.P., Stevenin, J., Lian, L.Y., Wilson, S.A., Allain, F.H.T.
EMBO J. (2006), 25, 5126-5137, PubMed 17036044 , DOI 10.1038/sj.emboj.7601385 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. RRM domain of SR rich factor 9G8, : 1 : 6 : 56 entities Detail

Interaction partners 1. RRM domain of SR rich factor 9G8, : 76 interactors Detail

Experiments performed 3 experiments Detail