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Backbone amide chemical shifts for iG80b E. coli RNase H
Authors
Butterwick, J.A., Palmer, A.G.
Assembly
iG80b ecRNH
Entity
1. iG80b ecRNH (polymer, Thiol state: all free), 156 monomers, 17653.83 Da Detail

MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD SQYVRQGITQ GWIHNWKKRG WKTADKKPVK NVDLWQRLDA ALGQHQIKWE WVKGHAGHPE NERCDELARA AAMNPTLEDT GYQVEV


Formula weight
17653.83 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 44.9 %, Completeness: 13.7 %, Completeness (bb): 29.7 % Detail

Polymer type: polypeptide(L)

Total1H15N
All13.7 % (155 of 1132) 8.9 % (85 of 950)38.5 % (70 of 182)
Backbone29.7 % (136 of 458)21.5 % (66 of 307)46.4 % (70 of 151)
Sidechain 2.8 % (19 of 674) 3.0 % (19 of 643) 0.0 % (0 of 31)
Aromatic 0.0 % (0 of 82) 0.0 % (0 of 76) 0.0 % (0 of 6)
Methyl 5.0 % (4 of 80) 5.0 % (4 of 80)

1. iG80b ecRNH

MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD SQYVRQGITQ GWIHNWKKRG WKTADKKPVK NVDLWQRLDA ALGQHQIKWE WVKGHAGHPE NERCDELARA AAMNPTLEDT GYQVEV

Sample

Temperature 300 (±1) K, pH 5.5 (±0.1)


#NameIsotope labelingTypeConcentration
1iG80b ecRNH[U-2H; U-15N]protein1.0 (±0.1) mM

Release date
2007-10-08
Citation
An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H
Butterwick, J.A., Palmer, A.G.
Protein Sci. (2006), 15, 2697-2707, PubMed 17088323 , DOI 10.1110/ps.062398606 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 7277 released on 2007-10-08
    Title Backbone amide chemical shifts for dG85 T. thermophilus RNase H
Related entities 1. iG80b ecRNH, : 1 : 1 : 188 entities Detail
Interaction partners 1. iG80b ecRNH, : 5 interactors Detail
Experiments performed 2 experiments Detail