BMRB: 7278

Backbone amide chemical shifts for iG80b E. coli RNase H

Authors

Butterwick, J.A., Palmer, A.G.

Assembly

iG80b ecRNH

Entity

1. iG80b ecRNH (polymer, Thiol state: all free), 156 monomers, 17653.83 Da Detail

MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD SQYVRQGITQ GWIHNWKKRG WKTADKKPVK NVDLWQRLDA ALGQHQIKWE WVKGHAGHPE NERCDELARA AAMNPTLEDT GYQVEV

Formula weight

17653.83 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 43.6 %, Completeness: 13.0 %, Completeness (bb): 29.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	13.0 % (146 of 1122)	8.2 % (78 of 950)	39.5 % (68 of 172)
Backbone	29.0 % (137 of 473)	21.4 % (69 of 322)	45.0 % (68 of 151)
Sidechain	1.5 % (10 of 649)	1.4 % (9 of 628)	4.8 % (1 of 21)
Aromatic	1.2 % (1 of 82)	1.3 % (1 of 76)	0.0 % (0 of 6)
Methyl	0.0 % (0 of 80)	0.0 % (0 of 80)

1. iG80b ecRNH

MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD SQYVRQGITQ GWIHNWKKRG WKTADKKPVK NVDLWQRLDA ALGQHQIKWE WVKGHAGHPE NERCDELARA AAMNPTLEDT GYQVEV

Show sample condition

Sample

Temperature 300 (±1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	iG80b ecRNH	[U-2H; U-15N]	protein	1.0 (±0.1) mM

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Release date

2007-10-08

Citation

An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H
Butterwick, J.A., Palmer, A.G.
Protein Sci. (2006), 15, 2697-2707, PubMed 17088323 , DOI 10.1110/ps.062398606 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. iG80b ecRNH, : 1 : 1 : 197 entities Detail

Interaction partners 1. iG80b ecRNH, : 7 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail