BMRB: 7307

Backbone assignment and RDCs of L11 in complex with RNA

Authors

Jonker, H., Ilin, S., Grimm, S., Wohnert, J., Schwalbe, H.

Assembly

L11-RNA complex

Entity

1. ribosomal L11 protein (polymer, Thiol state: all free), 141 monomers, 15088.73 Da Detail

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

2. 23S GAR RNA (polymer, Thiol state: not present), 60 monomers, 19437.57 Da Detail

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC

Total weight

34526.3 Da

Max. entity weight

19437.57 Da

Source organism

Thermotoga maritima , Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 68.2 %, Completeness: 24.0 %, Completeness (bb): 37.1 % Detail

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Polymer type: polypeptide(L) polyribonucleotide

	Total	1H	13C	15N
All	24.0 % (623 of 2594)	14.4 % (198 of 1379)	28.2 % (296 of 1048)	77.2 % (129 of 167)
Backbone	37.1 % (551 of 1486)	22.7 % (146 of 642)	38.7 % (276 of 713)	98.5 % (129 of 131)
Sidechain	7.3 % (90 of 1239)	7.1 % (52 of 737)	8.2 % (38 of 466)	0.0 % (0 of 36)
Aromatic	0.0 % (0 of 326)	0.0 % (0 of 163)	0.0 % (0 of 135)	0.0 % (0 of 28)
Methyl	18.8 % (33 of 176)	18.2 % (16 of 88)	19.3 % (17 of 88)

1. ribosomal L11 protein

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

2. 23S GAR RNA

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC

Show sample condition

Sample

Temperature 308 (±0.1) K, pH 6.1 (±0.05), Details binary complex

#	Name	Isotope labeling	Type	Concentration
1	ribosomal L11 protein	[U->95% 2D; U->98% 15N; U->98% 13C]	protein	0.3 mM
2	23S GAR RNA		RNA	0.5 mM
3	potassium phosphate		buffer	20 mM
4	potassium chloride		salt	200 mM
5	complete		protease inhibitor
6	superase		rnase inhibitor

Hide sample codition

RDC

127 RDC values in 1 lists
Field strength (¹H) 600 MHz, Temperature 298 (±0.1) K, pH 6.1 (±0.05) Detail

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Temperature 298 (±0.1) K, pH 6.1 (±0.05)

Experiment name IPAP_(1H,15N)HSQC

List #1 RDC_list_LR, RDC code 1DHNN, Field strength (¹H) 600 MHz, Details L11+RNA

Release date

2006-12-19

Citation

L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy
Jonker, H.R., Ilin, S., Grimm, S.K., Wohnert, J., Schwalbe, H.
Nucleic Acids Res. (2007), 35, 441-454, PubMed 17169991 , DOI 10.1093/nar/gkl1066 , Abstract

Related entities 1. ribosomal L11 protein, : 1 : 9 : 10 : 287 entities Detail

Related entities 2. 23S GAR RNA, : 100 : 6 entities Detail

Interaction partners 1. ribosomal L11 protein, : 1 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail