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Backbone assignment and RDCs of L11 in complex with RNA
Authors
Jonker, H., Ilin, S., Grimm, S., Wohnert, J., Schwalbe, H.
Assembly
L11-RNA complex
Entity
1. ribosomal L11 protein (polymer, Thiol state: all free), 141 monomers, 15088.73 Da Detail

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D


2. 23S GAR RNA (polymer, Thiol state: not present), 60 monomers, 19437.57 Da Detail

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC


Total weight
34526.3 Da
Max. entity weight
19437.57 Da
Source organism
Thermotoga maritima , Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts, RDCs
Chem. Shift Complete
Sequence coverage: 68.7 %, Completeness: 23.2 %, Completeness (bb): 36.2 % Detail

Polymer type: polypeptide(L) polyribonucleotide

Total1H13C15N
All23.2 % (603 of 2597)13.5 % (186 of 1379)27.5 % (288 of 1048)75.9 % (129 of 170)
Backbone36.2 % (535 of 1476)21.2 % (134 of 632)38.1 % (272 of 713)98.5 % (129 of 131)
Sidechain 6.2 % (78 of 1252) 7.1 % (53 of 747) 5.4 % (25 of 466) 0.0 % (0 of 39)
Aromatic 0.0 % (0 of 326) 0.0 % (0 of 163) 0.0 % (0 of 135) 0.0 % (0 of 28)
Methyl12.5 % (22 of 176)14.8 % (13 of 88)10.2 % (9 of 88)

1. ribosomal L11 protein

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

2. 23S GAR RNA

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC

Sample

Temperature 308 (±0.1) K, pH 6.1 (±0.05), Details binary complex


#NameIsotope labelingTypeConcentration
1ribosomal L11 protein[U->95% 2D; U->98% 15N; U->98% 13C]protein0.3 mM
223S GAR RNARNA0.5 mM
3potassium phosphatebuffer20 mM
4potassium chloridesalt200 mM
5completeprotease inhibitor
6superasernase inhibitor

RDC
127 RDC values in 1 lists
Field strength (1H) 600 MHz, Temperature 298 (±0.1) K, pH 6.1 (±0.05) Detail
Release date
2006-12-19
Citation
L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy
Jonker, H.R., Ilin, S., Grimm, S.K., Wohnert, J., Schwalbe, H.
Nucleic Acids Res. (2007), 35, 441-454, PubMed 17169991 , DOI 10.1093/nar/gkl1066 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 7308 released on 2006-09-27
    Title Backbone assignment and RDCs of L11 in complex with RNA and thiostrepton
Related entities 1. ribosomal L11 protein, : 1 : 9 : 10 : 209 entities Detail
Related entities 2. 23S GAR RNA, : 100 : 6 entities Detail
Interaction partners 1. ribosomal L11 protein, : 1 interactors Detail
Experiments performed 3 experiments Detail