BMRBj - BMREntryMaster

Found 1 Document (1.365 seconds)

BMRB: 7308

1C04

Backbone assignment and RDCs of L11 in complex with RNA and thiostrepton

Authors

Jonker, H., Ilin, S., Grimm, S., Wohnert, J., Schwalbe, H.

Assembly

L11-RNA-thiostrepton complex

Entity

1. ribosomal L11 protein (polymer, Thiol state: all free), 141 monomers, 15088.73 Da Detail

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

2. 23S GAR RNA (polymer, Thiol state: not present), 60 monomers, 19437.57 Da Detail

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC

3. Thiostrepton antibiotic (polymer, Thiol state: not available), 15 monomers, 1620.918 Da Detail

XTXXXXXIAX AXXXX

Total weight

36147.22 Da

Max. entity weight

19437.57 Da

Source organism

Thermotoga maritima , Escherichia coli , Streptomyces azureus

Exptl. method

NMR

Data set

RDCs, assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 63.4 %, Completeness: 24.7 %, Completeness (bb): 40.1 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	24.7 % (649 of 2631)	11.1 % (155 of 1396)	34.4 % (366 of 1064)	74.9 % (128 of 171)
Backbone	40.1 % (606 of 1510)	19.7 % (128 of 650)	48.3 % (350 of 725)	94.8 % (128 of 135)
Sidechain	11.2 % (141 of 1256)	3.6 % (27 of 746)	24.1 % (114 of 474)	0.0 % (0 of 36)
Aromatic	2.5 % (8 of 326)	3.1 % (5 of 163)	2.2 % (3 of 135)	0.0 % (0 of 28)
Methyl	13.4 % (25 of 186)	4.3 % (4 of 93)	22.6 % (21 of 93)

1. ribosomal L11 protein

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

2. 23S GAR RNA

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC

3. Thiostrepton antibiotic

XTXXXXXIAX AXX

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.1 (±0.05), Details ternary complex

#	Name	Isotope labeling	Type	Concentration
1	ribosomal L11 protein	[U->95% 2D; U->98% 15N; U->98% 13C]	protein	0.3 mM
2	23S GAR RNA		rna	0.3 mM
3	Thiostrepton antibiotic		antibiotic	0.3 mM
4	potassium phosphate		buffer	20 mM
5	potassium chloride		salt	200 mM
6	complete		protease inhibitoir
7	superase		rnase inhibitor

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 3.36 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 3.36 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 2.59 ppm, Outliers: 2 Detail

RDC

120 RDC values in 1 lists
Field strength (¹H) 600 MHz, Temperature 298 (±0.1) K, pH 6.1 (±0.05) Detail

Release date

2006-09-27

Citation

L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy
Jonker, H.R., Ilin, S., Grimm, S.K., Wohnert, J., Schwalbe, H.
Nucleic Acids Res. (2007), 35, 441-454, PubMed 17169991 , DOI 10.1093/nar/gkl1066 , Abstract

Related entities 1. ribosomal L11 protein, : 1 : 206 entities Detail

Related entities 2. 23S GAR RNA, : 1 : 99 : 6 entities Detail

Experiments performed 5 experiments Detail

1 2D (1H,15N)HSQC