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Backbone assignment and RDCs of L11 in complex with RNA and thiostrepton
Authors
Jonker, H., Ilin, S., Grimm, S., Wohnert, J., Schwalbe, H.
Assembly
L11-RNA-thiostrepton complex
Entity
1. ribosomal L11 protein (polymer, Thiol state: all free), 141 monomers, 15088.73 Da Detail

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D


2. 23S GAR RNA (polymer, Thiol state: not present), 60 monomers, 19437.57 Da Detail

GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC


3. Thiostrepton antibiotic (polymer, Thiol state: not available), 15 monomers, 1620.918 Da Detail

XTXXXXXIAX AXXXX


Total weight
36147.22 Da
Max. entity weight
19437.57 Da
Source organism
Thermotoga maritima , Escherichia coli , Streptomyces azureus
Exptl. method
NMR
Data set
RDCs, assigned_chemical_shifts
Chemcal Shifts
1H: 127, 13C: 354, 15N: 127 assignments Detail
IDEntity IDSeq IDComp IDAtom IDAtom typeValVal errAmbig. code
112ALACC171.01
212ALACAC48.51
312ALACBC15.81
413LYSHH8.301
513LYSCC173.31
613LYSCAC53.21
713LYSCBC30.21
813LYSNN121.41
914LYSHH8.721
1014LYSCC173.71
1114LYSCAC53.11
1214LYSCBC29.81
1314LYSNN124.21
1415VALHH8.561
1515VALCC173.01
1615VALCAC61.31
1715VALCBC29.41
1815VALNN126.21
1916ALAHH9.471
2016ALACC174.61
2116ALACAC49.41
2216ALANN131.31
2317ALAHH7.741
2417ALACC171.91
2517ALACAC48.71
2617ALACBC17.71
2717ALANN118.71
2818GLNHH8.111
2918GLNCC172.31
3018GLNCAC51.71
3118GLNCBC28.51
3218GLNNN118.51
3319ILEHH9.021
3419ILECC171.71
3519ILECAC57.11
3619ILECBC38.31
3719ILENN125.11
38110LYSHH8.771
39110LYSCC173.81
40110LYSCAC52.41
41110LYSCBC31.21
42110LYSNN128.41
43111LEUHH8.621
44111LEUCC172.61
45111LEUCAC50.41
46111LEUCBC43.11
47111LEUNN123.11
48112GLNHH8.841
49112GLNCC172.01
50112GLNCAC51.91
51112GLNCBC25.21
52112GLNNN122.01
53113LEUHH8.101
54113LEUCAC48.11
55113LEUCBC41.71
56113LEUNN123.41
57114PROCC173.91
58114PROCAC58.51
59115ALAHH8.211
60115ALACC176.51
61115ALACAC50.31
62115ALACBC16.51
63115ALANN127.71
64116GLYHH8.231
65116GLYCC171.21
66116GLYCAC42.31
67116GLYNN109.51
68117LYSHH7.631
69117LYSCC173.31
70117LYSCAC51.61
71117LYSCBC29.61
72117LYSNN120.61
73118ALAHH9.921
74118ALACC175.31
75118ALACAC50.51
76118ALACBC15.71
77118ALANN125.91
78119THRHH7.821
79119THRCAC56.71
80119THRCBC67.01
81119THRNN115.01
82120PROCC173.41
83121ALAHH7.141
84121ALACAC48.21
85121ALACBC12.91
86121ALANN121.81
87123PROCC174.41
88123PROCAC60.91
89124VALHH7.831
90124VALCC174.41
91124VALCAC64.11
92124VALCBC28.91
93124VALNN126.41
94125GLYHH6.601
95125GLYNN103.41
96126PROCC176.11
97127ALAHH6.891
98127ALACC176.61
99127ALACAC52.01
100127ALANN117.01
101128LEUHH7.561
102128LEUCC177.51
103128LEUCAC54.61
104128LEUNN115.31
105129GLYHH9.091
106129GLYCC174.81
107129GLYCAC43.81
108129GLYNN111.21
109130GLNHH7.521
110130GLNCC172.91
111130GLNCAC54.81
112130GLNNN119.01
113131HISHH6.961
114131HISCC171.81
115131HISCAC52.51
116131HISNN114.41
117132GLYHH7.661
118132GLYCC171.01
119132GLYCAC43.11
120132GLYNN108.11
121133VALHH7.121
122133VALCC172.41
123133VALCAC58.61
124133VALNN119.51
125134ASNHH8.511
126134ASNCC171.61
127134ASNCAC50.71
128134ASNCBC34.11
129134ASNNN124.71
130135ILEHH7.841
131135ILECC175.51
132135ILECAC62.11
133135ILENN129.91
134136METHH8.271
135136METCC176.41
136136METCAC54.31
137136METNN118.61
138137GLUHH7.911
139137GLUCC176.41
140137GLUCAC56.21
141137GLUCBC25.31
142137GLUNN121.71
143138PHEHH7.901
144138PHECC173.21
145138PHECAC58.91
146138PHECBC30.71
147138PHENN119.11
148139CYSHH8.151
149139CYSCC173.01
150139CYSCAC61.81
151139CYSNN117.01
152140LYSHH8.121
153140LYSCC177.31
154140LYSCAC56.71
155140LYSCBC28.61
156140LYSNN118.31
157141ARGHH7.841
158141ARGCC176.11
159141ARGCAC56.61
160141ARGNN119.71
161142PHEHH8.631
162142PHECC176.51
163142PHECAC59.21
164142PHECBC34.51
165142PHENN120.41
166143ASNHH8.721
167143ASNCC174.71
168143ASNCAC52.91
169143ASNCBC34.81
170143ASNNN121.61
171144ALAHH7.611
172144ALACC177.91
173144ALACAC51.61
174144ALACBC14.31
175144ALANN121.51
176145GLUHH7.541
177145GLUCC175.41
178145GLUCAC54.91
179145GLUCBC27.01
180145GLUNN115.71
181146THRHH7.161
182146THRCC172.51
183146THRCAC58.41
184146THRNN104.91
185147ALAHH7.051
186147ALACC176.11
187147ALACAC52.61
188147ALACBC15.21
189147ALANN126.11
190148ASPHH8.391
191148ASPCC173.91
192148ASPCAC51.61
193148ASPCBC36.11
194148ASPNN114.41
195149LYSHH7.571
196149LYSCC171.11
197149LYSCAC51.71
198149LYSCBC29.01
199149LYSNN121.51
200150ALAHH6.751
201150ALACC175.31
202150ALACAC51.21
203150ALACBC15.01
204150ALANN120.11
205151GLYHH9.061
206151GLYCC172.41
207151GLYCAC41.41
208151GLYNN112.61
209152METHH7.901
210152METCAC51.51
211152METCBC30.81
212152METNN118.11
213155PROCC173.01
214155PROCAC58.11
215155PROCBC28.51
216156VALHH8.981
217156VALCC172.71
218156VALCAC55.71
219156VALCBC32.21
220156VALNN121.61
221157VALHH8.421
222157VALCC174.61
223157VALCAC58.41
224157VALCBC29.31
225157VALNN125.41
226158ILEHH9.861
227158ILECC172.71
228158ILECAC57.61
229158ILECBC36.61
230158ILENN133.01
231159THRHH9.371
232159THRCC169.21
233159THRCAC60.11
234159THRCBC66.61
235159THRNN126.61
236160VALHH8.561
237160VALCC173.11
238160VALCAC57.41
239160VALCBC29.01
240160VALNN126.11
241161TYRHH8.751
242161TYRCC175.71
243161TYRCAC55.31
244161TYRCBC36.01
245161TYRNN127.01
246162GLUHH8.751
247162GLUCC173.21
248162GLUCAC56.11
249162GLUCBC25.81
250162GLUNN120.91
251163ASPHH7.701
252163ASPCC174.11
253163ASPCAC50.21
254163ASPCBC36.11
255163ASPNN117.31
256164LYSHH8.271
257164LYSCC172.91
258164LYSCAC56.21
259164LYSCBC24.81
260164LYSNN113.41
261165SERHH8.001
262165SERCC170.01
263165SERCAC56.11
264165SERCBC61.31
265165SERNN115.11
266166PHEHH8.191
267166PHECC172.21
268166PHECAC53.41
269166PHECBC39.01
270166PHENN112.81
271167THRHH9.091
272167THRCC170.31
273167THRCAC57.11
274167THRCBC69.41
275167THRNN113.21
276168PHEHH8.561
277168PHECC170.51
278168PHECAC53.31
279168PHECBC39.51
280168PHENN116.11
281169ILEHH8.271
282169ILECC171.61
283169ILECAC56.11
284169ILECBC38.51
285169ILENN117.41
286170ILEHH8.591
287170ILECC173.81
288170ILECAC57.21
289170ILECBC35.61
290170ILENN124.71
291171LYSHH8.531
292171LYSCC173.31
293171LYSCAC51.61
294171LYSCBC29.71
295171LYSNN128.41
296172THRHH8.391
297172THRCAC58.31
298172THRCBC66.11
299172THRNN111.01
300174PROCC175.01
301174PROCAC59.41
302175ALAHH9.871
303175ALACC177.21
304175ALACAC53.31
305175ALANN128.91
306176SERHH10.191
307176SERCC173.61
308176SERCAC57.31
309176SERCBC67.21
310176SERNN113.71
311177PHEHH6.741
312177PHECC174.31
313177PHECAC58.61
314177PHENN124.31
315178LEUHH8.061
316178LEUCC178.21
317178LEUCAC54.31
318178LEUCBC38.31
319178LEUNN119.21
320179LEUHH8.561
321179LEUCC175.31
322179LEUCAC55.31
323179LEUCBC38.61
324179LEUNN122.01
325180LYSHH7.571
326180LYSCC176.11
327180LYSCAC57.61
328180LYSNN119.51
329181LYSHH7.331
330181LYSCC177.31
331181LYSCAC54.51
332181LYSCBC27.51
333181LYSNN116.81
334182ALAHH7.831
335182ALACC175.81
336182ALACAC51.51
337182ALACBC15.01
338182ALANN122.81
339183ALAHH7.981
340183ALACC175.11
341183ALACAC48.81
342183ALACBC15.71
343183ALANN117.91
344184GLYHH7.751
345184GLYCC172.31
346184GLYCAC43.31
347184GLYNN109.21
348185ILEHH7.671
349185ILECC172.21
350185ILECAC56.11
351185ILECBC37.41
352185ILENN111.91
353186GLUHH8.401
354186GLUCC174.11
355186GLUCAC53.81
356186GLUCBC27.41
357186GLUNN119.01
358187LYSHH7.301
359187LYSCC173.71
360187LYSCAC51.21
361187LYSCBC32.91
362187LYSNN117.21
363188GLYHH8.961
364188GLYCC169.71
365188GLYCAC40.51
366188GLYNN107.61
367189SERHH7.721
368189SERCC174.51
369189SERCAC54.91
370189SERCBC62.21
371189SERNN109.81
372190SERHH8.641
373190SERCC172.51
374190SERCAC57.51
375190SERCBC62.41
376190SERNN121.91
377191GLUHH8.071
378191GLUCAC50.31
379191GLUCBC28.61
380191GLUNN120.11
381192PROCC171.71
382193LYSHH8.711
383193LYSCC174.41
384193LYSCAC52.41
385193LYSCBC30.91
386193LYSNN128.41
387194ARGHH7.881
388194ARGCC174.11
389194ARGCAC54.81
390194ARGCBC27.81
391194ARGNN119.51
392195LYSHH8.571
393195LYSCC171.91
394195LYSCAC52.11
395195LYSCBC30.11
396195LYSNN120.31
397196ILEHH8.671
398196ILECC175.61
399196ILECAC55.21
400196ILECBC32.51
401196ILENN129.31
402197VALHH9.071
403197VALCC172.81
404197VALCAC57.71
405197VALNN119.51
406198GLYHH7.511
407198GLYCC167.11
408198GLYCAC42.01
409198GLYNN108.01
410199LYSHH8.751
411199LYSCC172.11
412199LYSCAC51.51
413199LYSCBC32.71
414199LYSNN119.01
4151100VALHH8.511
4161100VALCC171.81
4171100VALCAC55.21
4181100VALCBC32.21
4191100VALNN113.71
4201101THRHH8.851
4211101THRCC174.71
4221101THRCAC56.71
4231101THRCBC68.71
4241101THRNN112.21
4251102ARGHH8.551
4261102ARGCC176.21
4271102ARGCAC56.71
4281102ARGCBC25.51
4291102ARGNN121.71
4301103LYSHH8.161
4311103LYSCC176.51
4321103LYSCAC55.51
4331103LYSCBC27.91
4341103LYSNN120.41
4351104GLNHH7.731
4361104GLNCC176.91
4371104GLNCAC56.41
4381104GLNNN119.91
4391105ILEHH8.041
4401105ILECC174.81
4411105ILECAC59.81
4421105ILECBC30.61
4431105ILENN120.31
4441106GLUHH8.341
4451106GLUCC175.01
4461106GLUCAC57.11
4471106GLUCBC26.21
4481106GLUNN122.71
4491107GLUHH7.841
4501107GLUCC177.41
4511107GLUCAC56.31
4521107GLUCBC26.01
4531107GLUNN118.51
4541108ILEHH8.181
4551108ILECC174.81
4561108ILECAC63.11
4571108ILECBC34.71
4581108ILENN122.41
4591109ALAHH8.811
4601109ALACC176.01
4611109ALACAC52.61
4621109ALACBC14.71
4631109ALANN123.11
4641110LYSHH8.211
4651110LYSCC177.31
4661110LYSCAC57.41
4671110LYSCBC28.91
4681110LYSNN115.51
4691111THRHH8.001
4701111THRCC172.81
4711111THRCAC63.81
4721111THRCBC65.41
4731111THRNN118.11
4741112LYSHH8.111
4751112LYSCC174.01
4761112LYSCAC53.01
4771112LYSNN115.41
4781113METHH7.431
4791113METCAC56.71
4801113METCBC26.41
4811113METNN121.11
4821114PROCC174.81
4831114PROCAC63.21
4841115ASPHH8.421
4851115ASPCC172.51
4861115ASPCAC51.41
4871115ASPCBC39.61
4881115ASPNN114.51
4891116LEUHH7.721
4901116LEUCC173.81
4911116LEUCAC49.81
4921116LEUCBC40.51
4931116LEUNN117.81
4941117ASNHH7.721
4951117ASNCC172.21
4961117ASNCAC48.51
4971117ASNCBC34.31
4981117ASNNN116.81
4991118ALAHH6.111
5001118ALACC174.31
5011118ALANN119.81
5021119ASNHH8.941
5031119ASNCC171.81
5041119ASNCAC49.01
5051119ASNCBC35.61
5061119ASNNN117.11
5071120SERHH7.221
5081120SERCC171.31
5091120SERCAC53.61
5101120SERCBC63.01
5111120SERNN111.31
5121121LEUHH8.901
5131121LEUCC175.61
5141121LEUCAC54.51
5151121LEUCBC37.51
5161121LEUNN124.91
5171122GLUHH8.861
5181122GLUCC176.51
5191122GLUCAC57.31
5201122GLUCBC25.01
5211122GLUNN119.41
5221123ALAHH7.521
5231123ALACC176.51
5241123ALACAC51.81
5251123ALANN121.61
5261124ALAHH7.781
5271124ALACC176.81
5281124ALACAC51.81
5291124ALACBC16.41
5301124ALANN120.31
5311125METHH8.551
5321125METCC175.41
5331125METCAC57.41
5341125METNN116.91
5351126LYSHH7.271
5361126LYSCC176.91
5371126LYSCAC57.31
5381126LYSCBC28.91
5391126LYSNN119.31
5401127ILEHH7.531
5411127ILECC174.31
5421127ILECAC62.91
5431127ILECBC35.21
5441127ILENN121.71
5451128ILEHH7.901
5461128ILECC177.01
5471128ILECAC58.91
5481128ILECBC28.31
5491128ILENN119.41
5501129GLUHH8.781
5511129GLUCC175.81
5521129GLUCAC57.11
5531129GLUCBC25.21
5541129GLUNN121.01
5551130GLYHH7.911
5561130GLYCC175.91
5571130GLYCAC44.51
5581130GLYNN108.31
5591131THRHH7.701
5601131THRCC175.61
5611131THRCAC64.01
5621131THRNN122.41
5631132ALAHH8.231
5641132ALACC176.41
5651132ALACAC54.11
5661132ALACBC12.01
5671132ALANN129.91
5681133LYSHH8.081
5691133LYSCC176.21
5701133LYSCAC56.31
5711133LYSCBC29.21
5721133LYSNN116.91
5731134SERHH7.371
5741134SERCC170.31
5751134SERCAC57.31
5761134SERNN116.51
5771135METHH7.301
5781135METCC173.81
5791135METCAC51.71
5801135METNN112.81
5811136GLYHH7.781
5821136GLYCC170.21
5831136GLYCAC43.91
5841136GLYNN108.41
5851137ILEHH7.281
5861137ILECC172.41
5871137ILECAC56.31
5881137ILECBC36.11
5891137ILENN118.81
5901138GLUHH8.331
5911138GLUCC171.61
5921138GLUCAC51.81
5931138GLUCBC28.21
5941138GLUNN128.51
5951139VALHH8.201
5961139VALCC174.01
5971139VALCAC58.21
5981139VALCBC28.21
5991139VALNN124.41
6001140VALHH8.811
6011140VALCC171.71
6021140VALCAC56.91
6031140VALCBC30.61
6041140VALNN125.21
6051141ASPHH7.901
6061141ASPCAC53.91
6071141ASPCBC38.31
6081141ASPNN125.91

LACS Plot; CA
Referencing offset: 3.36 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 3.36 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 2.59 ppm, Outliers: 2 Detail
RDC
120 RDC values in 1 lists
Field strength (1H) 600 MHz, Temperature 298 (±0.1) K, pH 6.1 (±0.05) Detail
Release date
2006-09-27
Citation
L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy
Jonker, H.R., Ilin, S., Grimm, S.K., Wohnert, J., Schwalbe, H.
Nucleic Acids Res. (2007), 35, 441-454, PubMed 17169991 , DOI 10.1093/nar/gkl1066 ,
Related entities 1. ribosomal L11 protein, : 1 : 128 entities Detail
Related entities 2. 23S GAR RNA, : 1 : 99 : 6 entities Detail
Experiments performed 5 experiments Detail